{"doi":"10.1039/d2lc01070d","title":"Mitigating neutrophil trafficking and cardiotoxicity with DS–IkL in a microphysiological system of a cytokine storm","abstract":", myocardium) are challenging to observe at a high spatial and temporal resolution in mouse models. In this study, we created a vascularized organ-on-a-chip system to mimic cytokine storm-like conditions and tested the effectiveness of a novel multivalent selectin-targeting carbohydrate conjugate (composed of DS - dermatan sulfate and IkL - a selectin-binding peptide, termed DS-IkL) in blocking infiltration of polymorphonuclear leukocytes (PMN). Our data shows that cytokine storm-like conditions induce endothelial cells to produce additional inflammatory cytokines and facilitate infiltration of PMNs into tissue. Treatment of tissues with DS-IkL (60 μM) reduced PMN accumulation in the tissue by >50%. We then created cytokine storm-like conditions in a vascularized cardiac tissue-chip and found that PMN infiltration increases the spontaneous beating rate of the cardiac tissue, and this effect is eliminated by treatment with DS-IkL (60 μM). In summary, we demonstrate the utility of an organ-on-a-chip platform to mimic COVID-19 related cytokine storm and that blocking leukocyte infiltration with DS-IkL could be a viable strategy to mitigate associated cardiac complications.","journal":"Lab on a Chip","year":2023,"id":371196,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9595,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":401356,"name":"Sergey Yechikov","orcid":null,"position":1,"is_corresponding":false},{"id":506274,"name":"Bhupinder Shergill","orcid":"0000-0002-7850-0000","position":2,"is_corresponding":false},{"id":407409,"name":"Tima Dehghani","orcid":"0000-0001-7563-2668","position":3,"is_corresponding":false},{"id":1130825,"name":"A Block","orcid":"0000-0003-0862-3627","position":4,"is_corresponding":false},{"id":252442,"name":"Harkanwalpreet Sodhi","orcid":"0000-0003-4614-503X","position":5,"is_corresponding":false},{"id":252443,"name":"Alyssa Panitch","orcid":"0000-0002-0360-1480","position":6,"is_corresponding":false},{"id":311780,"name":"Steven C. George","orcid":"0000-0003-2263-1914","position":7,"is_corresponding":false},{"id":311778,"name":"Venktesh S. Shirure","orcid":"0000-0002-4430-2612","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T01:15:45.330525Z","pmid":"37278194","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}