{"doi":"10.1039/d2cp00889k","title":"Characterizing dark state kinetics and single molecule fluorescence of FusionRed and FusionRed-MQ at low irradiances","abstract":"), which were previously unexplored experimental conditions. We characterized the kinetics of dark state transitions in these two FPs by using single molecule blinking and ensemble photobleaching experiments bridged with a dark state kinetic model. We find that at low irradiances, the recovery process to the ground state is minimally light-driven and FusionRed-MQ has a 1.3-fold longer ground state recovery time indicating a conformationally restricted dark-state chromophore in comparison to FusionRed. Our studies indicate that the brighter FusionRed-MQ variant exhibits higher dark state conversion rates with longer ground state recovery lifetimes, thus it is potentially a better candidate for SMLM applications than its progenitor FusionRed.","journal":"Physical Chemistry Chemical Physics","year":2022,"id":265109,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":495149,"name":"Connor Thomas","orcid":"0000-0002-6753-7123","position":1,"is_corresponding":false},{"id":924235,"name":"Ryan N. Wilson","orcid":null,"position":2,"is_corresponding":false},{"id":923681,"name":"Emma M. Simmerman","orcid":"0000-0003-3305-1165","position":3,"is_corresponding":false},{"id":495147,"name":"Sheng‐Ting Hung","orcid":"0000-0002-7081-2173","position":4,"is_corresponding":false},{"id":495150,"name":"Ralph Jimenez","orcid":"0000-0002-8989-405X","position":5,"is_corresponding":false},{"id":495146,"name":"Srijit Mukherjee","orcid":"0000-0002-6439-9802","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-19T00:26:45.905331Z","pmid":"35642612","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}