{"doi":"10.1039/d1sc05351e","title":"Oxime as a general photocage for the design of visible light photo-activatable fluorophores","abstract":"Photoactivatable fluorophores have been widely used for tracking molecular and cellular dynamics with subdiffraction resolution. In this work, we have prepared a series of photoactivatable probes using the oxime moiety as a new class of photolabile caging group in which the photoactivation process is mediated by a highly efficient photodeoximation reaction. Incorporation of the oxime caging group into fluorophores results in loss of fluorescence. Upon light irradiation in the presence of air, the oxime-caged fluorophores are oxidized to their carbonyl derivatives, restoring strong fluorophore fluorescence. To demonstrate the utility of these oxime-caged fluorophores, we have created probes that target different organelles for live-cell confocal imaging. We also carried out photoactivated localization microscopy (PALM) imaging under physiological conditions using low-power light activation in the absence of cytotoxic additives. Our studies show that oximes represent a new class of visible-light photocages that can be widely used for cellular imaging, sensing, and photo-controlled molecular release.","journal":"Chemical Science","year":2021,"id":159125,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9452,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":669349,"name":"Shichao Wang","orcid":"0000-0003-2171-9201","position":1,"is_corresponding":false},{"id":270659,"name":"Juan Tang","orcid":"0000-0002-1033-0666","position":2,"is_corresponding":false},{"id":669350,"name":"Vanessa Espinoza","orcid":"0000-0001-7265-9902","position":3,"is_corresponding":false},{"id":270661,"name":"Axel Loredo","orcid":"0000-0003-0144-8192","position":4,"is_corresponding":false},{"id":324117,"name":"Zeru Tian","orcid":"0000-0002-0437-2632","position":5,"is_corresponding":false},{"id":669351,"name":"R. Bruce Weisman","orcid":"0000-0001-8546-9980","position":6,"is_corresponding":false},{"id":270663,"name":"Han Xiao","orcid":"0000-0002-4311-971X","position":7,"is_corresponding":false},{"id":270660,"name":"Lushun Wang","orcid":"0000-0002-8373-2199","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-18T23:44:35.165422Z","pmid":"35003586","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}