{"doi":"10.1039/d1sc03508h","title":"A highly sensitive and selective fluoride sensor based on a riboswitch-regulated transcription coupled with CRISPR-Cas13a tandem reaction","abstract":"transcription and generate full-length transcribed RNA that can be recognized by CRISPR-Cas13a, triggering the collateral cleavage of the fluorophore-quencher labeled RNA probe and generating a fluorescence signal output. This tandem sensor can quantitatively detect fluoride at ambient temperature in aqueous solution with high sensitivity (limit of detection (LOD) ≈ 1.7 μM), high selectivity against other common anions, a wide dynamic range (0-800 μM) and a short sample-to-answer time (30 min). This work expands the application of nucleic acid sensors to negatively charged targets and demonstrates their potential for the on-site and real-time detection of fluoride in environmental monitoring and point-of-care diagnostics.","journal":"Chemical Science","year":2021,"id":165436,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":689036,"name":"Quanbing Mou","orcid":"0000-0001-6100-699X","position":1,"is_corresponding":false},{"id":689037,"name":"Peng Yan","orcid":"0000-0002-2127-4647","position":2,"is_corresponding":false},{"id":689038,"name":"Zhenglin Yang","orcid":"0000-0003-2743-5779","position":3,"is_corresponding":false},{"id":689039,"name":"Ying Xiong","orcid":"0000-0001-5943-1508","position":4,"is_corresponding":false},{"id":689982,"name":"Deyue Yan","orcid":null,"position":5,"is_corresponding":false},{"id":689040,"name":"Chuan Zhang","orcid":"0000-0002-9311-0799","position":6,"is_corresponding":false},{"id":689041,"name":"Xinyuan Zhu","orcid":"0000-0002-2891-837X","position":7,"is_corresponding":false},{"id":449411,"name":"Yi Lu","orcid":"0000-0003-1221-6709","position":8,"is_corresponding":false},{"id":689035,"name":"Yuan Ma","orcid":"0000-0002-0824-7310","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-18T23:45:40.838485Z","pmid":"34659710","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}