{"doi":"10.1039/d1cc05322a","title":"A self-immolative linker that releases thiols detects penicillin amidase and nitroreductase with high sensitivity <i>via</i> absorption spectroscopy","abstract":"enzymes. We demonstrate that thiocarbonate self-immolative linkers can be used to detect the enzymes penicillin G amidase (PGA) and nitroreductase (NTR) with high sensitivity using absorption spectroscopy. Paired with modern thiol amplification technology, the detection of PGA and NTR were achieved at concentrations of 160 nM and 52 nM respectively. In addition, the PGA probe was shown to be compatible with both biological thiols and enzymes present in cell lysates.","journal":"Chemical Communications","year":2022,"id":287221,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":320284,"name":"Joachim Justad Røise","orcid":"0000-0002-1794-7570","position":1,"is_corresponding":false},{"id":311049,"name":"Maomao He","orcid":"0000-0002-7682-1142","position":2,"is_corresponding":false},{"id":500740,"name":"I-Che Li","orcid":"0000-0002-5819-8094","position":3,"is_corresponding":false},{"id":967285,"name":"Alvin K. Agatep","orcid":null,"position":4,"is_corresponding":false},{"id":967286,"name":"Patrick Udenyi","orcid":null,"position":5,"is_corresponding":false},{"id":492016,"name":"Hesong Han","orcid":"0000-0001-5545-5238","position":6,"is_corresponding":false},{"id":748576,"name":"Nicole Jackson","orcid":"0000-0002-0202-3658","position":7,"is_corresponding":false},{"id":3209,"name":"D. Lucas Kerr","orcid":"0000-0002-0557-8341","position":8,"is_corresponding":false},{"id":966889,"name":"Dake Chen","orcid":"0000-0002-1966-1461","position":9,"is_corresponding":false},{"id":967287,"name":"Michael R. Stentzel","orcid":null,"position":10,"is_corresponding":false},{"id":755364,"name":"Emily Ruan","orcid":null,"position":11,"is_corresponding":false},{"id":486286,"name":"Lee W. Riley","orcid":"0000-0001-9916-3894","position":12,"is_corresponding":false},{"id":235839,"name":"Niren Murthy","orcid":"0000-0002-7815-7337","position":13,"is_corresponding":false},{"id":308924,"name":"Eli M. Espinoza","orcid":"0000-0002-0134-2833","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-19T00:29:56.781756Z","pmid":"35170593","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}