{"doi":"10.1039/d0nr07830a","title":"A simple, high-throughput method of protein and label removal from extracellular vesicle samples","abstract":"Evidence continues to increase of the clinical utility extracellular vesicles (EVs) as translational biomarkers. While a wide variety of EV isolation and purification methods have been implemented, few techniques are high-throughput and scalable for removing excess fluorescent reagents (e.g. dyes, antibodies). EVs are too small to be recovered from routine cell-processing procedures, such as filtration or centrifugation. The lack of suitable methods for removing unbound labels, especially in optical assays, is a major roadblock to accurate EV phenotyping and utilization of EV assays in a translational or clinical setting. Therefore, we developed a method for using a multi-modal resin, referred to as EV-Clean, to remove unbound labels from EV samples, and we demonstrate improvement in flow cytometric EV analysis with the use of this EV-Clean method.","journal":"Nanoscale","year":2021,"id":188211,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.948,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":576575,"name":"Bryce Killingsworth","orcid":null,"position":1,"is_corresponding":false},{"id":452561,"name":"Julia Kepley","orcid":"0000-0002-2458-5491","position":2,"is_corresponding":false},{"id":453727,"name":"Tim Traynor","orcid":null,"position":3,"is_corresponding":false},{"id":576576,"name":"Kathy McKinnon","orcid":null,"position":4,"is_corresponding":false},{"id":450067,"name":"Jason E. Savage","orcid":null,"position":5,"is_corresponding":false},{"id":576577,"name":"Deven Appel","orcid":null,"position":6,"is_corresponding":false},{"id":13200,"name":"Kenneth Aldape","orcid":"0000-0001-5119-7550","position":7,"is_corresponding":false},{"id":254789,"name":"Kevin Camphausen","orcid":"0000-0002-0450-4877","position":8,"is_corresponding":false},{"id":388227,"name":"Jay A. Berzofsky","orcid":"0000-0002-0342-3194","position":9,"is_corresponding":false},{"id":226931,"name":"Alexander R. Ivanov","orcid":"0000-0002-4691-8488","position":10,"is_corresponding":false},{"id":226792,"name":"Ionita Ghiran","orcid":"0000-0001-6655-3298","position":11,"is_corresponding":false},{"id":226807,"name":"Jennifer Jones","orcid":"0000-0002-9488-7719","position":12,"is_corresponding":false},{"id":215734,"name":"Joshua A Welsh","orcid":"0000-0002-1097-9756","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T23:49:05.976005Z","pmid":"33544111","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}