{"doi":"10.1039/d0lc01194k","title":"Investigations on T cell transmigration in a human skin-on-chip (SoC) model","abstract":"A microfluidics-based three-dimensional skin-on-chip (SoC) model is developed in this study to enable quantitative studies of transendothelial and transepithelial migration of human T lymphocytes in mimicked skin inflammatory microenvironments and to test new drug candidates. The keys results include 1) CCL20-dependent T cell transmigration is significantly inhibited by an engineered CCL20 locked dimer (CCL20LD), supporting the potential immunotherapeutic use of CCL20LD for treating skin diseases such as psoriasis; 2) transepithelial migration of T cells in response to a CXCL12 gradient mimicking T cell egress from the skin is significantly reduced by a sphingosine-1-phosphate (S1P) background, suggesting the role of S1P for T cell retention in inflamed skin tissues; and 3) T cell transmigration is induced by inflammatory cytokine stimulated epithelial cells in the SoC model. Collectively, the developed SoC model recreates a dynamic multi-cellular micro-environment that enables quantitative studies of T cell transmigration at a single cell level in response to physiological cutaneous inflammatory mediators and potential drugs.","journal":"Lab on a Chip","year":2021,"id":155945,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9494,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":369821,"name":"Anthony E. Getschman","orcid":"0000-0002-0143-1561","position":1,"is_corresponding":false},{"id":626483,"name":"Samuel Hwang","orcid":"0000-0001-7930-9964","position":2,"is_corresponding":false},{"id":275185,"name":"Brian F. Volkman","orcid":"0000-0002-6681-5179","position":3,"is_corresponding":false},{"id":659635,"name":"Thomas Klonisch","orcid":"0000-0003-0448-6741","position":4,"is_corresponding":false},{"id":659636,"name":"David B. Levin","orcid":"0000-0003-4533-500X","position":5,"is_corresponding":false},{"id":257941,"name":"Min Zhao","orcid":"0000-0002-2500-3035","position":6,"is_corresponding":false},{"id":660782,"name":"Susy Santos","orcid":null,"position":7,"is_corresponding":false},{"id":637800,"name":"Song Liu","orcid":"0000-0003-0301-9535","position":8,"is_corresponding":false},{"id":660783,"name":"Jasmine Cheng","orcid":null,"position":9,"is_corresponding":false},{"id":433871,"name":"Francis Lin","orcid":"0000-0003-1736-193X","position":10,"is_corresponding":false},{"id":433870,"name":"Xiaoou Ren","orcid":"0000-0002-6565-3454","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:44:08.368845Z","pmid":"33616124","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}