{"doi":"10.1039/d0cp04747c","title":"Capturing single molecules by nanopores: measured times and thermodynamics","abstract":"In numerous nanopore sensing applications transient interruptions in ion current through single nanopores induced by capturing solute molecules are a source of information on how solutes interact with the nanopores. We show that the distribution of time spent by a single captured solute molecule in a nanopore is bimodal with the majority of capture events being too fast to be experimentally resolved. As a result, the exact mean durations of the event and inter-event interval are orders of magnitude shorter than their measured values. Moreover, the exact and measured mean durations have qualitatively different dependences on the molecule diffusivity. This leads to a formal contradiction with the thermodynamics of molecule partitioning between the bulk and the nanopore. Here we resolve this controversy. We also demonstrate that, surprisingly, the probability of finding a molecule in the nanopore, obtained from the ratio of the measured mean durations of the capture event and interevent interval, is essentially identical to the exact equilibrium thermodynamic probability.","journal":"Physical Chemistry Chemical Physics","year":2021,"id":202022,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9468,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":345737,"name":"Sergey M. Bezrukov","orcid":"0000-0002-8209-8050","position":1,"is_corresponding":false},{"id":402165,"name":"Alexander M. Berezhkovskii","orcid":"0000-0002-0564-0408","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-18T23:51:05.955461Z","pmid":"33410847","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}