{"doi":"10.1039/b811673c","title":"Specificity of proteinlysinemethyltransferases and methods for detection oflysinemethylation of non-histoneproteins","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Post translational modification of histoneproteins including lysinemethylation is an important epigenetic mark, essential for gene regulation and development. Recently, several examples of lysinemethylation of non-histoneproteins have been discovered suggesting that this is a common post-translational modification for regulation of protein activity. Here, we review assays for the detection ofprotein methylation based on mass spectrometry, radiolabel and immunological approaches using protein and peptide substrates including application of SPOTpeptide arrays. Candidates for new methylation targets of protein methyltransferases can be predicted using the specificity of the enzyme and protein interaction data.</jats:p>\n                  <jats:p/>","journal":"Molecular BioSystems","year":2008,"id":668437,"datarank":0.5676284450877392,"base_score":3.784189633918261,"endowment":3.784189633918261,"self_citation_contribution":0.5676284450877392,"citation_network_contribution":0.0,"self_endowment_contribution":0.5676284450877392,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1745501,"name":"Arunkumar Dhayalan","orcid":null,"position":1,"is_corresponding":false},{"id":304765,"name":"Huimin Ma","orcid":"0000-0001-6155-9076","position":2,"is_corresponding":false},{"id":247179,"name":"Albert Jeltsch","orcid":"0000-0001-6113-9290","position":3,"is_corresponding":false},{"id":1745500,"name":"Philipp Rathert","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Specificity of proteinlysinemethyltransferases and methods for detection oflysinemethylation of non-histoneproteins","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Post translational modification of histoneproteins including lysinemethylation is an important epigenetic mark, essential for gene regulation and development. Recently, several examples of lysinemethylation of non-histoneproteins have been discovered suggesting that this is a common post-translational modification for regulation of protein activity. Here, we review assays for the detection ofprotein methylation based on mass spectrometry, radiolabel and immunological approaches using protein and peptide substrates including application of SPOTpeptide arrays. Candidates for new methylation targets of protein methyltransferases can be predicted using the specificity of the enzyme and protein interaction data.</jats:p>\n                  <jats:p/>","is_dataset_classified":null,"base_score":3.784189633918261,"endowment":3.784189633918261,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19396382","pmcid":null,"openalex_id":"https://openalex.org/W2058447361","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.08196721,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":2}],"oa_status":"closed","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://academic.oup.com/molecular-omics/article-pdf/4/12/1186/66204920/b811673c.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1039/b811673c","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19396382","host_type":"repository"}],"fields_of_study":["Epigenetics and DNA Methylation","Cancer-related gene regulation","RNA modifications and cancer","Histone-Lysine N-Methyltransferase","Histones","Lysine","Methylation","Protein Processing, Post-Translational","Proteins","Sensitivity and Specificity"],"mesh_terms":["Histones","Lysine","Methylation","Histone-Lysine N-Methyltransferase","Protein Processing, Post-Translational","Proteins","Sensitivity and Specificity"],"keywords":["Methyltransferase","Protein methylation","Methylation","Lysine","Histone methyltransferase","Histone","EZH2","Epigenetics","Biochemistry","Histone methylation","Biology","Histone H3","Peptide","Chemistry","DNA methylation","Gene","Gene expression","Amino acid"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-14T11:26:17.256113Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}