{"doi":"10.1038/srep45007","title":"Aminopeptidase N1 is involved in Bacillus thuringiensis Cry1Ac toxicity in the beet armyworm, Spodoptera exigua","abstract":"<jats:title>Abstract</jats:title><jats:p>Understanding how insecticidal proteins from the bacterium <jats:italic>Bacillus thuringiensis</jats:italic> (Bt) interact with their hosts is crucial to fully explain the molecular bases of Bt specificity and insecticidal activity. Previous studies support ATP binding cassette transporters (ABCC2/3) and one cadherin-like protein are Cry1Ac functional receptors in the beet armyworm (<jats:italic>Spodoptera exigua</jats:italic>). In this study, a combined one-dimensional gel electrophoresis and immunoblotting approach identified aminopeptidase N (APNs) as putative Cry1Ac binding proteins in the midgut brush border membrane of <jats:italic>S. exigua</jats:italic> larvae. Functional analyses by gene silencing of six different <jats:italic>S. exigua</jats:italic> APN genes (<jats:italic>SeAPN1, SeAPN2, SeAPN3, SeAPN4, SeAPN5</jats:italic> and <jats:italic>SeAPN6</jats:italic>) showed that only suppression of <jats:italic>SeAPN1</jats:italic> resulted in decreased larval susceptibility to Cry1Ac toxin. These results support that <jats:italic>SeAPN1</jats:italic> plays important functional role in Cry1Ac toxicity in <jats:italic>S. exigua</jats:italic>.</jats:p>","journal":"Scientific Reports","year":2017,"id":33137,"datarank":0.7398180059757893,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.38013371505603366,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.38013371505603366,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":9,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":174048,"name":"Songhe Cui","orcid":null,"position":1,"is_corresponding":false},{"id":174049,"name":"Lang Liu","orcid":null,"position":2,"is_corresponding":false},{"id":174050,"name":"Boyao Zhang","orcid":null,"position":3,"is_corresponding":false},{"id":174051,"name":"Weihua Ma","orcid":null,"position":4,"is_corresponding":false},{"id":162529,"name":"Xiaoping Wang","orcid":null,"position":5,"is_corresponding":false},{"id":174052,"name":"Chaoliang Lei","orcid":null,"position":6,"is_corresponding":false},{"id":174053,"name":"Lizhen Chen","orcid":null,"position":7,"is_corresponding":false},{"id":58013,"name":"Lin Qiu","orcid":"0000-0003-3389-3741","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28327568","pmcid":"PMC5361178","openalex_id":"https://openalex.org/W2597942363","authors":[],"funders":[],"total_grants":0,"fwci":0.5606,"citation_percentile":0.65584065,"influential_citations":0,"citation_trend":[{"year":2018,"count":2},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep45007.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep45007.pdf","host_type":"GOLD"},{"url":"https://www.nature.com/articles/srep45007.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep45007","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep45007","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28327568","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5361178","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5361178","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5361178?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Insect Resistance and Genetics","Entomopathogenic Microorganisms in Pest Control","Insect Pest Control Strategies","Medicine","Biology","Environmental Science","Aminopeptidases","Animals","Bacillus thuringiensis","Bacillus thuringiensis Toxins","Bacterial Proteins","Endotoxins","Gene Expression Regulation, Bacterial","Hemolysin Proteins","Larva","Protein Binding","Spodoptera"],"mesh_terms":["Bacillus thuringiensis Toxins","Aminopeptidases","Animals","Bacillus thuringiensis","Bacterial Proteins","Endotoxins","Hemolysin Proteins","Larva","Protein Binding","Gene Expression Regulation, Bacterial","Spodoptera"],"keywords":["Exigua","Cry1Ac","Spodoptera","Bacillus thuringiensis","Beet armyworm","Biology","Aminopeptidase","Midgut","Microbiology","ATP-binding cassette transporter","Biochemistry","Botany","Gene","Genetically modified crops","Larva","Transgene","Transporter","Amino acid","Bacteria","Genetics","Leucine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T15:43:40.233701Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}