{"doi":"10.1038/srep42167","title":"Role of E-type prostaglandin receptor EP3 in the vasoconstrictor activity evoked by prostacyclin in thromboxane-prostanoid receptor deficient mice","abstract":"<jats:title>Abstract</jats:title><jats:p>Prostacyclin, also termed as prostaglandin I<jats:sub>2</jats:sub>(PGI<jats:sub>2</jats:sub>), evokes contraction in vessels with limited expression of the prostacyclin receptor. Although the thromboxane-prostanoid receptor (TP) is proposed to mediate such a response of PGI<jats:sub>2</jats:sub>, other unknown receptor(s) might also be involved. TP knockout (TP<jats:sup>−/−</jats:sup>) mice were thus designed and used to test the hypothesis. Vessels, which normally show contraction to PGI<jats:sub>2</jats:sub>, were isolated for functional and biochemical analyses. Here, we showed that the contractile response evoked by PGI<jats:sub>2</jats:sub>was indeed only partially abolished in the abdominal aorta of TP<jats:sup>−/−</jats:sup>mice. Interestingly, further antagonizing the E-type prostaglandin receptor EP3 removed the remaining contractile activity, resulting in relaxation evoked by PGI<jats:sub>2</jats:sub>in such vessels of TP<jats:sup>−/−</jats:sup>mice. These results suggest that EP3 along with TP contributes to vasoconstrictor responses evoked by PGI<jats:sub>2</jats:sub>, and hence imply a novel mechanism for endothelial cyclooxygenase metabolites (which consist mainly of PGI<jats:sub>2</jats:sub>) in regulating vascular functions.</jats:p>","journal":"Scientific Reports","year":2017,"id":30081,"datarank":0.8121289524637948,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.31775342256314537,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.31775342256314537,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":12,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":162990,"name":"Yingzhan Zhang","orcid":null,"position":1,"is_corresponding":false},{"id":15500,"name":"Bin Liu","orcid":"0000-0002-9113-9694","position":2,"is_corresponding":false},{"id":164294,"name":"Wenhong Luo","orcid":null,"position":3,"is_corresponding":false},{"id":15834,"name":"Hui Li","orcid":"0000-0002-7879-1792","position":4,"is_corresponding":false},{"id":162994,"name":"Yingbi Zhou","orcid":null,"position":5,"is_corresponding":false},{"id":164293,"name":"Zhenhua Li","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28165064","pmcid":"PMC5292700","openalex_id":"https://openalex.org/W2586213198","authors":[],"funders":[],"total_grants":0,"fwci":2.1829,"citation_percentile":0.85947198,"influential_citations":3,"citation_trend":[{"year":2017,"count":2},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":4},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep42167.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep42167.pdf","host_type":"GOLD"},{"url":"https://www.nature.com/articles/srep42167.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep42167","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep42167","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28165064","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5292700","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5292700","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5292700?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Inflammatory mediators and NSAID effects","Eicosanoids and Hypertension Pharmacology","Nitric Oxide and Endothelin Effects","Chemistry","Medicine","Biology","Animals","Aorta, Abdominal","Base Sequence","Blood Pressure","Cyclooxygenase 2","Endothelium, Vascular","Epoprostenol","Female","Gene Expression Regulation","Male","Mice","Mice, Inbred C57BL","Mice, Knockout","Receptors, Prostaglandin E, EP3 Subtype","Receptors, Thromboxane","Renal Artery","Signal Transduction","Vasoconstriction","Vasoconstrictor Agents"],"mesh_terms":["Animals","Aorta, Abdominal","Base Sequence","Blood Pressure","Endothelium, Vascular","Female","Gene Expression Regulation","Male","Mice, Inbred C57BL","Epoprostenol","Renal Artery","Vasoconstriction","Vasoconstrictor Agents","Signal Transduction","Receptors, Thromboxane","Mice, Knockout","Mice","Cyclooxygenase 2","Receptors, Prostaglandin E, EP3 Subtype"],"keywords":["Prostacyclin","Prostanoid","Thromboxane receptor","Thromboxane","Prostaglandin","Endocrinology","Internal medicine","Receptor","Thromboxane A2","Contraction (grammar)","Cyclooxygenase","Chemistry","Biology","Medicine","Biochemistry","Platelet","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T01:46:02.208341Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}