{"doi":"10.1038/srep28820","title":"Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes","abstract":"<jats:title>Abstract</jats:title><jats:p>Desmosomes are adhesion plaques that mediate cell-cell adhesion in many tissues, including the epidermis, and generate mechanical resistance to tissues. The extracellular domains of desmosomal cadherin proteins, desmogleins and desmocollins, are required for the interaction with cadherins of the neighbouring cells, whereas their cytoplasmic tails associate with cytoplasmic proteins which mediate connection to intermediate filaments. Disruption of desmosomal adhesion by mutations, autoantibodies or bacterial toxins results in severe human disorders of e.g. the skin and the heart. Despite the vital role of desmosomes in various tissues, the details of their molecular assembly are not clear. We here show that the two members of the flotillin protein family directly interact with the cytoplasmic tails of desmogleins. Depletion of flotillins in human keratinocytes results in weakened desmosomal adhesion and reduced expression of desmoglein-3, most likely due to a reduction in the desmosomal pool due to increased turnover. In the absence of flotillins, desmoglein-3 shows an altered localisation pattern in the cell-cell junctions of keratinocytes, which is highly similar to the localisation observed upon treatment with <jats:italic>pemphigus vulgaris</jats:italic> autoantibodies. Thus, our data show that flotillins, which have previously been connected to the classical cadherins, are also of importance for the desmosomal cell adhesion.</jats:p>","journal":"Scientific Reports","year":2016,"id":43124,"datarank":1.105479019853901,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.585618634433942,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.585618634433942,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":21,"citers_with_citation_signal":16,"citers_with_endowment":16,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":205455,"name":"Jawahir Ali","orcid":null,"position":1,"is_corresponding":false},{"id":205456,"name":"Nina Kurrle","orcid":null,"position":2,"is_corresponding":false},{"id":205457,"name":"Yvonne Exner","orcid":null,"position":3,"is_corresponding":false},{"id":205458,"name":"Rüdiger Eming","orcid":null,"position":4,"is_corresponding":false},{"id":205459,"name":"Michael Hertl","orcid":null,"position":5,"is_corresponding":false},{"id":205460,"name":"Antje Banning","orcid":null,"position":6,"is_corresponding":false},{"id":205461,"name":"Ritva Tikkanen","orcid":null,"position":7,"is_corresponding":false},{"id":205454,"name":"Frauke Völlner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27346727","pmcid":"PMC4922016","openalex_id":"https://openalex.org/W2472943400","authors":[],"funders":[],"total_grants":0,"fwci":4.8695,"citation_percentile":0.95438588,"influential_citations":0,"citation_trend":[{"year":2016,"count":4},{"year":2017,"count":4},{"year":2018,"count":6},{"year":2019,"count":6},{"year":2020,"count":1},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep28820.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep28820.pdf","host_type":"GOLD"},{"url":"https://www.nature.com/articles/srep28820.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep28820","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep28820","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27346727","host_type":"repository"},{"url":"http://geb.uni-giessen.de/geb/volltexte/2017/12463/","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4922016","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4922016","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4922016?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Autoimmune Bullous Skin Diseases","Caveolin-1 and cellular processes","Wnt/β-catenin signaling in development and cancer","Medicine","Biology","Animals","Autoantibodies","Cadherins","Cell Adhesion","Cell Line, Tumor","Cytoplasm","Cytoskeletal Proteins","Desmoglein 3","Desmosomes","Epidermis","Humans","Keratinocytes","Membrane Proteins","Mice","Mice, Knockout","Microscopy, Confocal","Pemphigus","Protein Domains","RNA, Small Interfering","Rats"],"mesh_terms":["Protein Domains","Animals","Autoantibodies","Cell Adhesion","Cytoplasm","Cytoskeletal Proteins","Desmosomes","Epidermis","Humans","Membrane Proteins","Pemphigus","Keratinocytes","Cadherins","Mice, Knockout","Microscopy, Confocal","RNA, Small Interfering","Cell Line, Tumor","Desmoglein 3","Mice","Rats"],"keywords":["Desmoglein","Desmosome","Cadherin","Plakoglobin","Cell biology","Pemphigus vulgaris","Desmoglein 3","Cell adhesion","Cytoplasm","Desmoglein 1","Adhesion","Pemphigus","Biology","Cell adhesion molecule","Chemistry","Cell","Catenin","Immunology","Autoantibody","Signal transduction","Genetics","Antibody"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T17:14:18.350813Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}