{"doi":"10.1038/srep08508","title":"No difference in small bowel microbiota between patients with irritable bowel syndrome and healthy controls","abstract":"<jats:title>Abstract</jats:title><jats:p>Several studies have indicated that colonic microbiota may exhibit important differences between patients with irritable bowel syndrome (IBS) and healthy controls. Less is known about the microbiota of the small bowel. We used massive parallel sequencing to explore the composition of small bowel mucosa-associated microbiota in patients with IBS and healthy controls. We analysed capsule biopsies from the jejunum of 35 patients (26 females) with IBS aged 18-(36)-57 years and 16 healthy volunteers (11 females) aged 20-(32)-48 years. Sequences were analysed based on taxonomic classification. The phyla with the highest total abundance across all samples were: Firmicutes (43%), Proteobacteria (23%), Bacteroidetes (15%), Actinobacteria (9.3%) and Fusobacteria (7.0%). The most abundant genera were: <jats:italic>Streptococcus</jats:italic> (19%), <jats:italic>Veillonella</jats:italic> (13%), <jats:italic>Prevotella</jats:italic> (12%), <jats:italic>Rothia</jats:italic> (6.4%), <jats:italic>Haemophilus</jats:italic> (5.7%), <jats:italic>Actinobacillus</jats:italic> (5.5%), <jats:italic>Escherichia</jats:italic> (4.6%) and <jats:italic>Fusobacterium</jats:italic> (4.3%). We found no difference among major phyla or genera between patients with IBS and controls. We identified a cluster of samples in the small bowel microbiota dominated by <jats:italic>Prevotella</jats:italic>, which may represent a common enterotype of the upper small intestine. The remaining samples formed a gradient, dominated by <jats:italic>Streptococcus</jats:italic> at one end and <jats:italic>Escherichia</jats:italic> at the other.</jats:p>","journal":"Scientific Reports","year":2015,"id":636894,"datarank":0.6628260911694899,"base_score":4.418840607796598,"endowment":4.418840607796598,"self_citation_contribution":0.6628260911694899,"citation_network_contribution":0.0,"self_endowment_contribution":0.6628260911694899,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":82,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1653420,"name":"Björn Winckler","orcid":null,"position":1,"is_corresponding":false},{"id":1653424,"name":"Elin Lundin","orcid":null,"position":2,"is_corresponding":false},{"id":1653426,"name":"Katherina Zakikhany","orcid":null,"position":3,"is_corresponding":false},{"id":1653428,"name":"Gunnar Sandström","orcid":null,"position":4,"is_corresponding":false},{"id":386042,"name":"Weimin Ye","orcid":"0000-0002-6859-4648","position":5,"is_corresponding":false},{"id":539470,"name":"Lars Engstrand","orcid":"0000-0002-7713-2373","position":6,"is_corresponding":false},{"id":539466,"name":"Greger Lindberg","orcid":"0000-0002-2432-2720","position":7,"is_corresponding":false},{"id":539474,"name":"Aldona Dlugosz","orcid":"0000-0003-3540-2571","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"No difference in small bowel microbiota between patients with irritable bowel syndrome and healthy controls","abstract":"<jats:title>Abstract</jats:title><jats:p>Several studies have indicated that colonic microbiota may exhibit important differences between patients with irritable bowel syndrome (IBS) and healthy controls. Less is known about the microbiota of the small bowel. We used massive parallel sequencing to explore the composition of small bowel mucosa-associated microbiota in patients with IBS and healthy controls. We analysed capsule biopsies from the jejunum of 35 patients (26 females) with IBS aged 18-(36)-57 years and 16 healthy volunteers (11 females) aged 20-(32)-48 years. Sequences were analysed based on taxonomic classification. The phyla with the highest total abundance across all samples were: Firmicutes (43%), Proteobacteria (23%), Bacteroidetes (15%), Actinobacteria (9.3%) and Fusobacteria (7.0%). The most abundant genera were: <jats:italic>Streptococcus</jats:italic> (19%), <jats:italic>Veillonella</jats:italic> (13%), <jats:italic>Prevotella</jats:italic> (12%), <jats:italic>Rothia</jats:italic> (6.4%), <jats:italic>Haemophilus</jats:italic> (5.7%), <jats:italic>Actinobacillus</jats:italic> (5.5%), <jats:italic>Escherichia</jats:italic> (4.6%) and <jats:italic>Fusobacterium</jats:italic> (4.3%). We found no difference among major phyla or genera between patients with IBS and controls. We identified a cluster of samples in the small bowel microbiota dominated by <jats:italic>Prevotella</jats:italic>, which may represent a common enterotype of the upper small intestine. The remaining samples formed a gradient, dominated by <jats:italic>Streptococcus</jats:italic> at one end and <jats:italic>Escherichia</jats:italic> at the other.</jats:p>","is_dataset_classified":null,"base_score":4.418840607796598,"endowment":4.418840607796598,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25687743","pmcid":"PMC4330528","openalex_id":"https://openalex.org/W1964406472","authors":[],"funders":[],"total_grants":0,"fwci":4.1317,"citation_percentile":0.94782281,"influential_citations":0,"citation_trend":[{"year":2015,"count":3},{"year":2016,"count":10},{"year":2017,"count":9},{"year":2018,"count":8},{"year":2019,"count":6},{"year":2020,"count":11},{"year":2021,"count":6},{"year":2022,"count":11},{"year":2023,"count":8},{"year":2024,"count":4},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep08508.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep08508.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep08508","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep08508","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25687743","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4330528","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4330528","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4330528?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Gut microbiota and health","Gastrointestinal motility and disorders","Clostridium difficile and Clostridium perfringens research","Adolescent","Adult","Biodiversity","Case-Control Studies","Cluster Analysis","Female","Humans","Intestine, Small","Irritable Bowel Syndrome","Male","Metagenome","Microbiota","Middle Aged","Principal Component Analysis","Young Adult"],"mesh_terms":["Adolescent","Adult","Female","Humans","Intestine, Small","Male","Middle Aged","Cluster Analysis","Case-Control Studies","Principal Component Analysis","Irritable Bowel Syndrome","Biodiversity","Metagenome","Young Adult","Microbiota"],"keywords":["Fusobacteria","Irritable bowel syndrome","Veillonella","Firmicutes","Prevotella","Fusobacterium","Bacteroidetes","Biology","Gastroenterology","Streptococcus","Gut flora","Internal medicine","Bacteroides","Microbiology","Medicine","Immunology","Bacteria","16S ribosomal RNA","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T17:54:54.872858Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}