{"doi":"10.1038/srep07935","title":"Identification of novel viral receptors with cell line expressing viral receptor-binding protein","abstract":"<jats:title>Abstract</jats:title><jats:p>The viral cell receptors and infection can be blocked by the expression of the viral receptor-binding protein. Thus, the viral cell receptor is an attractive target for anti-viral strategies and the identification of viral cell receptor is critical for better understanding and controlling viral disease. As a model system for viral entry and anti-retroviral approaches, avian sarcoma/leukosis virus (ASLV, including the A-J ten subgroups) has been studied intensively and many milestone discoveries have been achieved based on work with ASLV. Here, we used a DF1 cell line expressed viral receptor-binding protein to efficiently identify chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus ALV-J (avian leukosis virus subgroup J). Our data demonstrate that antibodies or siRNA to chANXA2 significantly inhibited ALV-J infection and replication and over-expression of chANXA2 permitted the entry of ALV-J into its non-permissible cells. Our findings have not only identified chANXA2 as a novel biomarker for anti-ALV-J, but also demonstrated that cell lines with the expression of viral receptor-binding protein could be as efficient tools for isolating functional receptors to identify novel anti-viral targets.</jats:p>","journal":"Scientific Reports","year":2015,"id":17280,"datarank":1.0177679647033457,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":0.5179372881770651,"self_endowment_contribution":0.49983067652628066,"citer_contribution":0.5179372881770651,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":15,"citers_with_citation_signal":15,"citers_with_endowment":15,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":124171,"name":"Jianqiang Ye","orcid":null,"position":1,"is_corresponding":false},{"id":124172,"name":"Aijian Qin","orcid":null,"position":2,"is_corresponding":false},{"id":88171,"name":"Lin Wang","orcid":"0000-0002-4905-3432","position":3,"is_corresponding":false},{"id":124173,"name":"Xuming Hu","orcid":null,"position":4,"is_corresponding":false},{"id":124174,"name":"Kun Qian","orcid":null,"position":5,"is_corresponding":false},{"id":124175,"name":"Hongxia Shao","orcid":null,"position":6,"is_corresponding":false},{"id":124170,"name":"Mei Mei","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.332204510175204,"endowment":3.332204510175204,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25604889","pmcid":"PMC4300512","openalex_id":"https://openalex.org/W2003076324","authors":[],"funders":[],"total_grants":0,"fwci":2.0829,"citation_percentile":0.86735312,"influential_citations":2,"citation_trend":[{"year":2016,"count":3},{"year":2017,"count":5},{"year":2018,"count":7},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":4},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep07935.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep07935.pdf","host_type":"GOLD"},{"url":"https://www.nature.com/articles/srep07935.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep07935","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep07935","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25604889","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4300512","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4300512","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4300512?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["interferon and immune responses","Virus-based gene therapy research","Herpesvirus Infections and Treatments","Medicine","Biology","Alpharetrovirus","Animals","Annexin A2","Avian Proteins","Chickens","HEK293 Cells","Humans","Receptors, Virus"],"mesh_terms":["Animals","Alpharetrovirus","Chickens","Humans","Receptors, Virus","Annexin A2","Avian Proteins","HEK293 Cells"],"keywords":["Viral entry","Receptor","Biology","Viral protein","Virology","Viral replication","Cell culture","Retrovirus","Viral structural protein","Receptor expression","Virus","Molecular biology","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T18:12:59.663389Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}