{"doi":"10.1038/srep02866","title":"The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation","abstract":"RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3'-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.","journal":"Scientific Reports","year":2013,"id":8527,"datarank":0.5983476069846413,"base_score":3.9889840465642745,"endowment":3.9889840465642745,"self_citation_contribution":0.5983476069846413,"citation_network_contribution":0.0,"self_endowment_contribution":0.5983476069846413,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0425,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2013-10-09","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":74168,"name":"Akira Iwase","orcid":"0000-0003-3294-7939","position":1,"is_corresponding":false},{"id":74169,"name":"Thomas Gänsewig","orcid":null,"position":2,"is_corresponding":false},{"id":74170,"name":"Alexander Sherstnev","orcid":null,"position":3,"is_corresponding":false},{"id":74171,"name":"Céline Duc","orcid":"0000-0001-7426-4541","position":4,"is_corresponding":false},{"id":33805,"name":"Geoffrey J. Barton","orcid":"0000-0002-9014-5355","position":5,"is_corresponding":false},{"id":74172,"name":"Kousuke Hanada","orcid":"0000-0001-8854-4261","position":6,"is_corresponding":false},{"id":74173,"name":"Mieko Higuchi-Takeuchi","orcid":null,"position":7,"is_corresponding":false},{"id":74174,"name":"Minami Matsui","orcid":"0000-0001-5162-2668","position":8,"is_corresponding":false},{"id":74175,"name":"Keiko Sugimoto","orcid":"0000-0002-9209-8230","position":9,"is_corresponding":false},{"id":58087,"name":"Kemal Kazan","orcid":"0000-0002-5169-8934","position":10,"is_corresponding":false},{"id":7298,"name":"Gordon G. Simpson","orcid":"0000-0001-6744-5889","position":11,"is_corresponding":false},{"id":61833,"name":"Ken Shirasu","orcid":"0000-0002-0349-3870","position":12,"is_corresponding":false},{"id":74176,"name":"Mieko Higuchi‐Takeuchi","orcid":null,"position":13,"is_corresponding":false},{"id":74167,"name":"Rebecca Lyons","orcid":null,"position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}