{"doi":"10.1038/srep02320","title":"Force produced after stretch in sarcomeres and half-sarcomeres isolated from skeletal muscles","abstract":"<jats:title>Abstract</jats:title><jats:p>The goal of this study was to evaluate if isolated sarcomeres and half-sarcomeres produce a long-lasting increase in force after a stretch is imposed during activation. Single and half-sarcomeres were isolated from myofibrils using micro-needles, which were also used for force measurements. After full force development, both preparations were stretched by different magnitudes. The sarcomere length (SL) or half-sarcomere length variations (HSL) were extracted by measuring the initial and final distances from the Z-line to the adjacent Z-line or to a region externally adjacent to the M-line of the sarcomere, respectively. Half-sarcomeres generated approximately the same amount of isometric force (29.0 ± SD 15.5 nN·μm<jats:sup>−2</jats:sup>) as single sarcomeres (32.1 ± SD 15.3 nN·μm<jats:sup>−2</jats:sup>) when activated. In both cases, the steady-state forces after stretch were higher than the forces during isometric contractions at similar conditions. The results suggest that stretch-induced force enhancement is partly caused by proteins within the half-sarcomere.</jats:p>","journal":"Scientific Reports","year":2013,"id":598241,"datarank":0.5641800173540344,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"self_citation_contribution":0.5641800173540344,"citation_network_contribution":0.0,"self_endowment_contribution":0.5641800173540344,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1532854,"name":"Bruno M. Baroni","orcid":null,"position":1,"is_corresponding":false},{"id":869586,"name":"José A. Correa","orcid":"0000-0002-9975-6670","position":2,"is_corresponding":false},{"id":1532856,"name":"Marco A. Vaz","orcid":null,"position":3,"is_corresponding":false},{"id":1532858,"name":"Dilson E. Rassier","orcid":null,"position":4,"is_corresponding":false},{"id":1532853,"name":"Fábio C. Minozzo","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Force produced after stretch in sarcomeres and half-sarcomeres isolated from skeletal muscles","abstract":"<jats:title>Abstract</jats:title><jats:p>The goal of this study was to evaluate if isolated sarcomeres and half-sarcomeres produce a long-lasting increase in force after a stretch is imposed during activation. Single and half-sarcomeres were isolated from myofibrils using micro-needles, which were also used for force measurements. After full force development, both preparations were stretched by different magnitudes. The sarcomere length (SL) or half-sarcomere length variations (HSL) were extracted by measuring the initial and final distances from the Z-line to the adjacent Z-line or to a region externally adjacent to the M-line of the sarcomere, respectively. Half-sarcomeres generated approximately the same amount of isometric force (29.0 ± SD 15.5 nN·μm<jats:sup>−2</jats:sup>) as single sarcomeres (32.1 ± SD 15.3 nN·μm<jats:sup>−2</jats:sup>) when activated. In both cases, the steady-state forces after stretch were higher than the forces during isometric contractions at similar conditions. The results suggest that stretch-induced force enhancement is partly caused by proteins within the half-sarcomere.</jats:p>","is_dataset_classified":null,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23900500","pmcid":"PMC3728588","openalex_id":"https://openalex.org/W2086880343","authors":[],"funders":[],"total_grants":0,"fwci":2.4685,"citation_percentile":0.8842082,"influential_citations":0,"citation_trend":[{"year":2014,"count":3},{"year":2015,"count":6},{"year":2016,"count":5},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":4},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/srep02320.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/srep02320.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/srep02320","host_type":"publisher"},{"url":"https://doi.org/10.1038/srep02320","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23900500","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3728588","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3728588","host_type":"repository"},{"url":"http://hdl.handle.net/10183/250034","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3728588","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3728588?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Muscle activation and electromyography studies","Muscle Physiology and Disorders","Cardiomyopathy and Myosin Studies","Animals","Cells, Cultured","Elastic Modulus","Isometric Contraction","Physical Stimulation","Rabbits","Sarcomeres","Stress, Mechanical"],"mesh_terms":["Animals","Cells, Cultured","Isometric Contraction","Physical Stimulation","Rabbits","Sarcomeres","Stress, Mechanical","Elastic Modulus"],"keywords":["Sarcomere","Anatomy","Skeletal muscle","Biology","Medicine","Myocyte","Internal medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T15:20:47.452366Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}