{"doi":"10.1038/sj.leu.2401154","title":"A novel single cell PCR assay: detection of human T lymphotropic virus type I DNA in lymphocytes of patients with adult T cell leukemia","abstract":null,"journal":"Leukemia","year":1998,"id":46611,"datarank":1.3248094725564017,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.9952257859559687,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.9952257859559687,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":8,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":215899,"name":"K Murakami","orcid":null,"position":1,"is_corresponding":false},{"id":215900,"name":"T Sawada","orcid":null,"position":2,"is_corresponding":false},{"id":215901,"name":"H Taguchi","orcid":null,"position":3,"is_corresponding":false},{"id":215902,"name":"I Miyoshi","orcid":null,"position":4,"is_corresponding":false},{"id":215898,"name":"T Miyagi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A novel single cell PCR assay: detection of human T lymphotropic virus type I DNA in lymphocytes of patients with adult T cell leukemia","abstract":"The abnormal lymphocytes in adult T cell leukemia (ATL) reveal a peculiar morphology that is characterized by indented or lobulated nuclei. While human T lymphotropic virus type I (HTLV-I) is thought to be integrated in ATL cells, the correlation between the nuclear irregularities and HTLV-I infection is obscure. We have devised a novel single cell polymerase chain reaction (PCR) technique to examine the integration of HTLV-I provirus genome in cells from two patients with ATL. To isolate single cells, peripheral blood smears were prepared on thin polyester slides and stained with May-Grünwald-Giemsa. Morphologically defined single cells were cut out after light microscopy. The HTLV-I DNA sequences were detected not only in ATL cells but also in normal-looking lymphocytes. This novel PCR method may provide a valuable tool for understanding the molecular events associated with HTLV-I infection at the single cell level.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9766512","pmcid":null,"openalex_id":"https://openalex.org/W2317793112","authors":[],"funders":[],"total_grants":0,"fwci":0.2247,"citation_percentile":0.52917812,"influential_citations":0,"citation_trend":[{"year":2016,"count":1}],"oa_status":"closed","license":"https://www.springer.com/tdm","oa_locations":[{"url":"https://www.nature.com/articles/2401154.pdf","host_type":"BRONZE"},{"url":"https://www.nature.com/articles/2401154","host_type":"publisher"},{"url":"https://doi.org/10.1038/sj.leu.2401154","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9766512","host_type":"repository"}],"fields_of_study":["T-cell and Retrovirus Studies","Animal Disease Management and Epidemiology","Vector-Borne Animal Diseases","Medicine","Biology","Adult","DNA Primers","DNA, Viral","Human T-lymphotropic virus 1","Humans","Leukemia-Lymphoma, Adult T-Cell","Lymphocytes","Polymerase Chain Reaction","Tumor Cells, Cultured"],"mesh_terms":["Adult","DNA, Viral","Humans","Lymphocytes","Tumor Cells, Cultured","Human T-lymphotropic virus 1","Leukemia-Lymphoma, Adult T-Cell","Polymerase Chain Reaction","DNA Primers"],"keywords":["Provirus","Polymerase chain reaction","Biology","Virology","Human T-lymphotropic virus 1","T-cell leukemia","Leukemia","Virus","Molecular biology","Cell","Giemsa stain","DNA","Jurkat cells","Cell type","T cell","Immunology","Genome","Gene","Genetics","Immune system"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-12T02:42:49.502123Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}