{"doi":"10.1038/sj.icb.7100128","title":"Perlecan: a major IL‐2‐binding proteoglycan in murine spleen","abstract":"<jats:p>Although interleukin‐2 (IL‐2) is typically considered a soluble cytokine, our laboratory has shown that the availability of IL‐2 in lymphoid tissues is regulated, in part, by an association with heparan sulfate glycosaminoglycan. Heparan sulfate is usually found in proteoglycan form, in which the heparan sulfate chains are covalently linked to a specific core protein. We now show that perlecan is one of the major IL‐2‐binding heparan sulfate proteoglycans in murine spleen. IL‐2 binds perlecan via heparan sulfate chains, as enzymatic removal of heparan sulfate from splenic perlecan abolishes its ability to bind IL‐2. Furthermore, we demonstrate that perlecan‐bound IL‐2 supports the proliferation of an IL‐2‐dependent cell line. Identification of perlecan as a major heparan sulfate proteoglycan that binds IL‐2 has implications for both the localization and regulation of IL‐2 <jats:italic>in vivo</jats:italic>.</jats:p>","journal":"Immunology &amp; Cell Biology","year":2008,"id":36897,"datarank":1.2419245724972094,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.8083688088127847,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.8083688088127847,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":13,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":185987,"name":"Elliott T Stevens","orcid":null,"position":1,"is_corresponding":false},{"id":185989,"name":"Deandra R Smith","orcid":null,"position":2,"is_corresponding":false},{"id":185991,"name":"Thomas N Wight","orcid":null,"position":3,"is_corresponding":false},{"id":185992,"name":"Lucile E Wrenshall","orcid":null,"position":4,"is_corresponding":false},{"id":185985,"name":"John D Miller","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18040286","pmcid":null,"openalex_id":"https://openalex.org/W2085399604","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"R01 A147825","title":null},{"funder_name":"National Institutes of Health","grant_id":"18645","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI47825","title":null}],"total_grants":3,"fwci":0.2571,"citation_percentile":0.56866928,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1038%2Fsj.icb.7100128","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1038/sj.icb.7100128","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1038/sj.icb.7100128","host_type":"publisher"},{"url":"https://doi.org/10.1038/sj.icb.7100128","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18040286","host_type":"repository"},{"url":"https://corescholar.libraries.wright.edu/ncbp/1024","host_type":"journal"},{"url":"https://works.bepress.com/lucile_wrenshall/51","host_type":"journal"}],"fields_of_study":["Proteoglycans and glycosaminoglycans research","Glycosylation and Glycoproteins Research","Cell Adhesion Molecules Research","Chemistry","Medicine","Biology","Animals","Cell Proliferation","Heparan Sulfate Proteoglycans","Humans","Interleukin-2","Mice","Spleen"],"mesh_terms":["Animals","Humans","Interleukin-2","Spleen","Heparan Sulfate Proteoglycans","Cell Proliferation","Mice"],"keywords":["Perlecan","Heparan sulfate","Proteoglycan","Glycosaminoglycan","Chemistry","Biochemistry","Syndecan 1","Cell biology","Molecular biology","Extracellular matrix","Biology","Cell"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T17:47:46.810203Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}