{"doi":"10.1038/sj.gt.3301899","title":"Monitoring adenoviral DNA delivery, using a mutant herpes simplex virus type 1 thymidine kinase gene as a PET reporter gene","abstract":null,"journal":"Gene Therapy","year":2002,"id":637021,"datarank":0.5742962094733643,"base_score":3.828641396489095,"endowment":3.828641396489095,"self_citation_contribution":0.5742962094733643,"citation_network_contribution":0.0,"self_endowment_contribution":0.5742962094733643,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":45,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1653761,"name":"K Nguyen","orcid":null,"position":1,"is_corresponding":false},{"id":1516856,"name":"N Satyamurthy","orcid":null,"position":2,"is_corresponding":false},{"id":1653765,"name":"J R Barrio","orcid":null,"position":3,"is_corresponding":false},{"id":1653768,"name":"M E Phelps","orcid":null,"position":4,"is_corresponding":false},{"id":1653771,"name":"S S Gambhir","orcid":null,"position":5,"is_corresponding":false},{"id":1653773,"name":"H R Herschman","orcid":null,"position":6,"is_corresponding":false},{"id":1653759,"name":"Q Liang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Monitoring adenoviral DNA delivery, using a mutant herpes simplex virus type 1 thymidine kinase gene as a PET reporter gene","abstract":"Current gene therapy protocols often suffer from an inability to monitor the site, level and persistence of gene expression following somatic DNA delivery. Herpes simplex virus 1 thymidine kinase (HSV1-tk) is currently under intensive investigation as a reporter gene for in vivo imaging of reporter gene expression. The presence of the HSV1-tk reporter gene is repetitively and non-invasively monitored by systemic injection of positron-emitting, radionuclide-labeled thymidine analogues or acycloguanosine HSV1-TK substrates and subsequent detection, by positron emission tomography, of trapped, phosphorylated product. To improve the efficacy of the HSV1-tk PET reporter gene system, both alternative substrates and mutations in the HSV1-tk gene have been described. We used a replication defective adenovirus to deliver the HSV1-sr39tk mutant enzyme and the wild-type HSV1-tk enzyme to mice. HSV1-sr39TK demonstrates greater sensitivity than wild-type HSV1-TK enzyme in vivo, using 9-[(4-[(18)F]fluoro-3-hydroxymethylbutyl)guanine as probe, following adenovirus-mediated hepatic expression in mice. Using this adenoviral delivery system, the location, magnitude and duration of HSV1-sr39tk PET reporter gene expression could be non-invasively, quantitatively and repetitively monitored for over 3 months by microPET.","is_dataset_classified":null,"base_score":3.828641396489095,"endowment":3.828641396489095,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12457279","pmcid":null,"openalex_id":"https://openalex.org/W2331638268","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01 CA84572","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P50 CA86306","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA82214-01","title":null},{"funder_name":"National Institutes of Health","grant_id":"2P50CA086306-11","title":"Molecular Imaging of DNA Salvage Metabolism in Cancer"}],"total_grants":4,"fwci":3.2052,"citation_percentile":0.92157387,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2021,"count":2}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"https://www.nature.com/articles/3301899.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/3301899","host_type":"publisher"},{"url":"https://doi.org/10.1038/sj.gt.3301899","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12457279","host_type":"repository"},{"url":"https://dx.doi.org/10.1038/sj.gt.3301899","host_type":""}],"fields_of_study":["Virus-based gene therapy research","Herpesvirus Infections and Treatments","Viral Infectious Diseases and Gene Expression in Insects","0301 basic medicine","03 medical and health sciences","0302 clinical medicine","Adenoviridae","Animals","Cell Line","DNA","Fluorine Radioisotopes","Gene Expression","Genes, Reporter","Genetic Vectors","Herpesvirus 1, Human","Liver","Male","Mice","Mice, Inbred Strains","Mutation","Radiopharmaceuticals","Thymidine Kinase","Tomography, Emission-Computed"],"mesh_terms":["Adenoviridae","Animals","Cell Line","DNA","Fluorine Radioisotopes","Genetic Vectors","Liver","Male","Mice, Inbred Strains","Mutation","Thymidine Kinase","Tomography, Emission-Computed","Gene Expression","Genes, Reporter","Herpesvirus 1, Human","Radiopharmaceuticals","Mice"],"keywords":["Thymidine kinase","Reporter gene","Genetic enhancement","Herpes simplex virus","Molecular biology","Gene delivery","Biology","Gene expression","Mutant","Gene product","Adenoviridae","Gene","Virology","Virus","Biochemistry","Male","Fluorine Radioisotopes","Genetic Vectors","Mice, Inbred Strains","DNA","Herpesvirus 1, Human","Cell Line","Mice","Liver","Genes, Reporter","Mutation","Animals","Radiopharmaceuticals","Tomography, Emission-Computed"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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