{"doi":"10.1038/s42003-024-06961-5","title":"Loss-of-function in testis-specific serine/threonine protein kinase triggers male infertility in an invasive moth","abstract":"Genetic biocontrol technologies present promising and eco-friendly strategies for the management of pest and insect-transmitted diseases. Although considerable advancements achieve in gene drive applications targeting mosquitoes, endeavors to combat agricultural pests have been somewhat restricted. Here, we identify that the testis-specific serine/threonine kinases (TSSKs) family is uniquely expressed in the testes of Cydia pomonella, a prominent global invasive species. We further generated male moths with disrupted the expression of TSSKs and those with TSSKs disrupted using RNA interference and CRISPR/Cas9 genetic editing techniques, resulting in significant disruptions in spermiogenesis, decreased sperm motility, and hindered development of eggs. Further explorations into the underlying post-transcriptional regulatory mechanisms reveales the involvement of lnc117962 as a competing endogenous RNA (ceRNA) for miR-3960, thereby regulating TSSKs. Notably, orchard trials demonstrates that the release of male strains can effectively suppress population growth. Our findings indicate that targeting TSSKs could serve as a feasible avenue for managing C. pomonella populations, offering significant insights and potential strategies for controlling invasive pests through genetic sterile insect technique (gSIT) technology.","journal":"Communications Biology","year":2024,"id":425241,"datarank":0.6528069638452859,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.1961285981867724,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.1961285981867724,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":16,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9604,"is_data_producer":true,"deposit_databanks":{"BioProject":["PRJNA1102100"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":912441,"name":"Yaqi Wang","orcid":"0000-0003-0001-9687","position":1,"is_corresponding":false},{"id":1223348,"name":"Kang-Wu Zheng","orcid":null,"position":2,"is_corresponding":false},{"id":1222854,"name":"Zhiping Wang","orcid":"0000-0002-1172-9230","position":3,"is_corresponding":false},{"id":1222855,"name":"Ronghua Liu","orcid":"0000-0002-7521-0913","position":4,"is_corresponding":false},{"id":145860,"name":"Pengcheng Wang","orcid":"0000-0001-6043-4132","position":5,"is_corresponding":false},{"id":1222856,"name":"Yuting Li","orcid":"0000-0001-8231-4262","position":6,"is_corresponding":false},{"id":112284,"name":"Ping Gao","orcid":null,"position":7,"is_corresponding":false},{"id":262214,"name":"Omar S. Akbari","orcid":"0000-0002-6853-9884","position":8,"is_corresponding":false},{"id":1222857,"name":"Xue Yang","orcid":"0000-0002-3919-8013","position":9,"is_corresponding":false},{"id":345564,"name":"Zihan Wei","orcid":"0000-0001-9234-3497","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T01:58:26.927596Z","pmid":"39363033","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}