{"doi":"10.1038/s42003-024-06823-0","title":"Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to pneumococcal vaccination","abstract":"Single-cell RNA sequencing (scRNA-seq) can resolve transcriptional features from individual cells, but scRNA-seq techniques capable of resolving the variable regions of B cell receptors (BCRs) remain limited, especially from widely-used 3'-barcoded libraries. Here, we report a method that can recover paired, full-length variable region sequences of BCRs from 3'-barcoded scRNA-seq libraries. We first verify this method (B3E-seq) can produce accurate, full-length BCR sequences. We then apply this method to profile B cell responses elicited against the capsular polysaccharide of Streptococcus pneumoniae serotype 3 (ST3) by glycoconjugate vaccines in five infant rhesus macaques. We identify BCR features associated with specificity for the ST3 antigen which are present in multiple vaccinated monkeys, indicating a convergent response to vaccination. These results demonstrate the utility of our method to resolve key features of the B cell repertoire and profile antigen-specific responses elicited by vaccination.","journal":"Communications Biology","year":2024,"id":441216,"datarank":0.3758495814442181,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.030461817495111152,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.030461817495111152,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":6,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9524,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE232873","GSE232873"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1020756,"name":"Yiming J. Zhang","orcid":"0000-0002-8102-3815","position":1,"is_corresponding":false},{"id":816867,"name":"Jin Hwan Kim","orcid":"0000-0002-1632-2421","position":2,"is_corresponding":false},{"id":1175906,"name":"MaryAnn Murillo","orcid":null,"position":3,"is_corresponding":false},{"id":1175907,"name":"Suddham Singh","orcid":null,"position":4,"is_corresponding":false},{"id":616798,"name":"Jakob Loschko","orcid":null,"position":5,"is_corresponding":false},{"id":1175575,"name":"Naveen Surendran","orcid":"0000-0003-3811-7282","position":6,"is_corresponding":false},{"id":740940,"name":"Ognjen Sekulović","orcid":"0000-0003-1006-0595","position":7,"is_corresponding":false},{"id":1016019,"name":"Ellie Feng","orcid":"0009-0002-4900-4814","position":8,"is_corresponding":false},{"id":1253663,"name":"Shuting Shi","orcid":"0000-0001-8377-1625","position":9,"is_corresponding":false},{"id":236278,"name":"Darrell J. Irvine","orcid":"0000-0002-8637-1405","position":10,"is_corresponding":false},{"id":256892,"name":"Sarita U. Patil","orcid":null,"position":11,"is_corresponding":false},{"id":615044,"name":"Isis Kanevsky","orcid":"0000-0001-9173-9377","position":12,"is_corresponding":false},{"id":745612,"name":"Laurent Chorro","orcid":null,"position":13,"is_corresponding":false},{"id":63891,"name":"John Christopher Love","orcid":"0000-0003-0921-3144","position":14,"is_corresponding":false},{"id":633121,"name":"Duncan M. Morgan","orcid":"0000-0002-3253-1362","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-19T02:01:06.281460Z","pmid":"39341987","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}