{"doi":"10.1038/s42003-024-06579-7","title":"Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry","abstract":"The rapid evolution of mass spectrometry-based single-cell proteomics now enables the cataloging of several thousand proteins from single cells. We investigated whether we could discover cellular heterogeneity beyond proteome, encompassing post-translational modifications (PTM), protein-protein interaction, and variants. By optimizing the mass spectrometry data interpretation strategy to enable the detection of PTMs and variants, we have generated a high-definition dataset of single-cell and nuclear proteomic-states. The data demonstrate the heterogeneity of cell-states and signaling dependencies at the single-cell level and reveal epigenetic drug-induced changes in single nuclei. This approach enables the exploration of previously uncharted single-cell and organellar proteomes revealing molecular characteristics that are inaccessible through RNA profiling.","journal":"Communications Biology","year":2024,"id":424257,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9172,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":664387,"name":"Firdous Ahmad Bhat","orcid":"0000-0003-3632-6718","position":1,"is_corresponding":false},{"id":1220594,"name":"Neha Joshi","orcid":"0000-0001-9232-1589","position":2,"is_corresponding":false},{"id":1220595,"name":"Letícia Sandoval","orcid":"0000-0002-6916-2249","position":3,"is_corresponding":false},{"id":765065,"name":"Husheng Ding","orcid":null,"position":4,"is_corresponding":false},{"id":1221165,"name":"Anu Jain","orcid":null,"position":5,"is_corresponding":false},{"id":54150,"name":"Jane A. Peterson","orcid":null,"position":6,"is_corresponding":false},{"id":485313,"name":"Taewook Kang","orcid":"0000-0003-3959-4777","position":7,"is_corresponding":false},{"id":1221166,"name":"Ganesh P. Pujari","orcid":null,"position":8,"is_corresponding":false},{"id":1189847,"name":"Jennifer L. Tomlinson","orcid":"0000-0002-0909-3165","position":9,"is_corresponding":false},{"id":1216170,"name":"Rohit Budhraja","orcid":"0000-0002-4209-2180","position":10,"is_corresponding":false},{"id":964110,"name":"Roman M. Zenka","orcid":"0000-0002-8337-2314","position":11,"is_corresponding":false},{"id":581728,"name":"Nagarajan Kannan","orcid":"0000-0002-8825-2178","position":12,"is_corresponding":false},{"id":554733,"name":"Benjamin R. Kipp","orcid":"0000-0001-9477-0118","position":13,"is_corresponding":false},{"id":235319,"name":"Surendra Dasari","orcid":"0000-0002-7972-3556","position":14,"is_corresponding":false},{"id":267594,"name":"Alexandre Gaspar‐Maia","orcid":"0000-0001-8528-5575","position":15,"is_corresponding":false},{"id":230197,"name":"Rory L. Smoot","orcid":"0000-0001-8543-661X","position":16,"is_corresponding":false},{"id":297824,"name":"Richard K. Kandasamy","orcid":"0000-0002-0894-1222","position":17,"is_corresponding":false},{"id":60408,"name":"Akhilesh Pandey","orcid":"0000-0001-9943-6127","position":18,"is_corresponding":false},{"id":554731,"name":"Dong‐Gi Mun","orcid":"0000-0003-3588-3440","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:58:07.191194Z","pmid":"39030393","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}