{"doi":"10.1038/s42003-023-04495-w","title":"Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons","abstract":"Botulinum neurotoxins (BoNTs) have been widely used clinically as a muscle relaxant. These toxins target motor neurons and cleave proteins essential for neurotransmitter release like Synaptosomal-associated protein of 25 kDa (SNAP-25). In vitro assays for BoNT testing using rodent cells or immortalized cell lines showed limitations in accuracy and physiological relevance. Here, we report a cell-based assay for detecting SNAP-25-cleaving BoNTs by combining human induced Pluripotent Stem Cells (hiPSC)-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase. This assay is convenient, rapid, free-of-specialized antibodies, with a detection sensitivity of femtomolar concentrations of toxin, and can be used to study the different steps of BoNT intoxication.","journal":"Communications Biology","year":2023,"id":330692,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":342361,"name":"Feifan Yu","orcid":"0000-0002-3205-0040","position":1,"is_corresponding":false},{"id":1055571,"name":"Sylvain Roquevière","orcid":null,"position":2,"is_corresponding":false},{"id":1054964,"name":"Juliette Duchesne de Lamotte","orcid":"0000-0002-0546-3886","position":3,"is_corresponding":false},{"id":821257,"name":"Johannes Krupp","orcid":"0000-0001-8120-2728","position":4,"is_corresponding":false},{"id":298483,"name":"Min Dong","orcid":"0000-0002-1744-7293","position":5,"is_corresponding":false},{"id":821258,"name":"Camille Nicoleau","orcid":"0000-0002-9803-1053","position":6,"is_corresponding":false},{"id":821256,"name":"Laurent Cotter","orcid":"0000-0003-1095-1056","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T01:09:14.691473Z","pmid":"36717690","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}