{"doi":"10.1038/s42003-022-03423-8","title":"Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat","abstract":"Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. However, research and development has been hindered by the lack of serum-free media that enable the robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described for pluripotent stem cells. Here, B8 is adapted for bovine satellite cells through the addition of a single component, recombinant albumin, which renders it suitable for long-term satellite cell expansion without sacrificing myogenicity. This new media (Beefy-9) maintains cell growth over the entire period tested (seven passages), with an average doubling time of 39 h. Along with demonstrated efficacy for bovine cells, Beefy-9 offers a promising starting-point for developing serum-free media for other meat-relevant species. Ultimately, this work offers a foundation for escaping cultured meat research's reliance on serum, thereby accelerating the field.","journal":"Communications Biology","year":2022,"id":232027,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":290,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9473,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":303245,"name":"Addison B. Mirliani","orcid":null,"position":1,"is_corresponding":false},{"id":842756,"name":"Miriam L. Rittenberg","orcid":null,"position":2,"is_corresponding":false},{"id":842757,"name":"Michelle Shub","orcid":null,"position":3,"is_corresponding":false},{"id":805944,"name":"Eugene C. White","orcid":null,"position":4,"is_corresponding":false},{"id":256655,"name":"John S.K. Yuen","orcid":"0000-0001-9854-1654","position":5,"is_corresponding":false},{"id":225373,"name":"David L. Kaplan","orcid":"0000-0002-9245-7774","position":6,"is_corresponding":false},{"id":256654,"name":"Andrew J. Stout","orcid":"0000-0001-9470-1032","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T00:21:12.348693Z","pmid":"35654948","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}