{"doi":"10.1038/s42003-021-01802-1","title":"Structure of membrane diacylglycerol kinase in lipid bilayers","abstract":"Diacylglycerol kinase (DgkA) is a small integral membrane protein, responsible for the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Its structures reported in previous studies, determined in detergent micelles by solution NMR and in monoolein cubic phase by X-ray crystallography, differ significantly. These differences point to the need to validate these detergent-based structures in phospholipid bilayers. Here, we present a well-defined homo-trimeric structure of DgkA in phospholipid bilayers determined by magic angle spinning solid-state NMR (ssNMR) spectroscopy, using an approach combining intra-, inter-molecular paramagnetic relaxation enhancement (PRE)-derived distance restraints and CS-Rosetta calculations. The DgkA structure determined in lipid bilayers is different from the solution NMR structure. In addition, although ssNMR structure of DgkA shows a global folding similar to that determined by X-ray, these two structures differ in monomeric symmetry and dynamics. A comparative analysis of DgkA structures determined in three different detergent/lipid environments provides a meaningful demonstration of the influence of membrane mimetic environments on the structure and dynamics of membrane proteins.","journal":"Communications Biology","year":2021,"id":197494,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9415,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":487168,"name":"Yang Shen","orcid":"0000-0003-1408-8034","position":1,"is_corresponding":false},{"id":771509,"name":"Yanke Chen","orcid":"0000-0001-7863-7755","position":2,"is_corresponding":false},{"id":589385,"name":"Zhengfeng Zhang","orcid":"0000-0003-2025-8327","position":3,"is_corresponding":false},{"id":771510,"name":"Shaojie Ma","orcid":"0000-0002-7951-0104","position":4,"is_corresponding":false},{"id":323931,"name":"Qianfen Wan","orcid":"0000-0003-2483-0087","position":5,"is_corresponding":false},{"id":546891,"name":"Qiong Tong","orcid":"0009-0001-9348-9065","position":6,"is_corresponding":false},{"id":486470,"name":"Clemens Glaubitz","orcid":"0000-0002-3554-6586","position":7,"is_corresponding":false},{"id":771511,"name":"Maili Liu","orcid":"0000-0002-9359-915X","position":8,"is_corresponding":false},{"id":589387,"name":"Jun Yang","orcid":"0000-0002-4480-5340","position":9,"is_corresponding":false},{"id":722281,"name":"Jianping Li","orcid":"0000-0002-2334-6456","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T23:50:23.532351Z","pmid":"33674677","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}