{"doi":"10.1038/s42003-020-0896-2","title":"Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis","abstract":"Single-cell omics provide insight into cellular heterogeneity and function. Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. Here we show the utility of this technology in mouse and human model systems for as many as 50 antibodies to targeted proteins and, separately, >70 targeted RNA regions. We demonstrate that this method can be applied to multi-modal protein and RNA analyses. It can be scaled by expansion of the split-pool process and effectively renders sequencing instruments as versatile multi-parameter flow cytometers.","journal":"Communications Biology","year":2020,"id":76356,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":398038,"name":"Felice-Alessio Bava","orcid":"0000-0002-7052-1659","position":1,"is_corresponding":false},{"id":398894,"name":"Mary Shen","orcid":null,"position":2,"is_corresponding":false},{"id":398039,"name":"Carolina Dallett","orcid":"0000-0002-6034-1099","position":3,"is_corresponding":false},{"id":398895,"name":"Sri Paladugu","orcid":null,"position":4,"is_corresponding":false},{"id":107289,"name":"Nikolay Samusik","orcid":"0000-0002-3559-1010","position":5,"is_corresponding":false},{"id":398896,"name":"Simon Yu","orcid":null,"position":6,"is_corresponding":false},{"id":398897,"name":"Razika Hussein","orcid":null,"position":7,"is_corresponding":false},{"id":398898,"name":"Grantland Hillman","orcid":null,"position":8,"is_corresponding":false},{"id":398899,"name":"Samuel G. Higgins","orcid":null,"position":9,"is_corresponding":false},{"id":398900,"name":"Melanie Lou","orcid":null,"position":10,"is_corresponding":false},{"id":398901,"name":"Angelica Trejo","orcid":null,"position":11,"is_corresponding":false},{"id":398902,"name":"Laura Qin","orcid":null,"position":12,"is_corresponding":false},{"id":398040,"name":"Yu Chuan Tai","orcid":"0000-0002-3976-4820","position":13,"is_corresponding":false},{"id":107288,"name":"Shigemi Kinoshita","orcid":null,"position":14,"is_corresponding":false},{"id":398903,"name":"Astraea Jager","orcid":null,"position":15,"is_corresponding":false},{"id":398904,"name":"Deval Lashkari","orcid":null,"position":16,"is_corresponding":false},{"id":55258,"name":"Yury Goltsev","orcid":"0000-0003-4079-7969","position":17,"is_corresponding":false},{"id":398905,"name":"Sedide Öztürk","orcid":null,"position":18,"is_corresponding":false},{"id":35731,"name":"Garry P. Nolan","orcid":"0000-0002-8862-9043","position":19,"is_corresponding":false},{"id":36087,"name":"Maeve O’Huallachain","orcid":"0000-0002-0618-6032","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:47:46.469293Z","pmid":"32382044","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}