{"doi":"10.1038/s41598-024-68656-5","title":"Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004–2022) belong to two SS14-like clusters","abstract":"Syphilis is a multistage sexually transmitted disease caused by Treponema pallidum ssp. pallidum. In the Czech Republic, there are around 700-800 new syphilis cases annually, continuously increasing since 2012. This study analyzed a total of 1228 samples from 2004 to 2022. Of the PCR-positive typeable samples (n = 415), 68.7% were fully-typed (FT), and 31.3% were partially-typed. Most of the identified isolates belonged to the SS14-clade and only 6.3% were the Nichols-like cluster. While in the beginning of sample collection isolates have been macrolide-susceptible, recent isolates are completely resistant to macrolides. Among the FT samples, 34 different allelic profiles (APs) were found. Most of the profiles (n = 27) appeared just once in the Czech population, while seven profiles were detected more than twice. The most frequent APs belonged to two separate groups of SS14-like isolates, including group of 1.3.1 (ST 1) and 1.26.1 (ST 25) profiles, and the second group containing 1.1.8 (ST 3), 1.1.1 (ST 2), and 1.1.3 (ST 11) (representing 57.5%, and 25.3% of all detected APs, respectively). Both groups consistently differed in 6 nucleotide positions in five genes (TP0150, TP0324, TP0515, TP0548, and TP0691) coding amino-acid replacements suggesting that one or more of these differences could be involved in the higher success of the first group.","journal":"Scientific Reports","year":2024,"id":453780,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8432,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":486382,"name":"Petra Pospíšilová","orcid":"0000-0002-4732-5051","position":1,"is_corresponding":false},{"id":1277065,"name":"Eliška Dastychová","orcid":null,"position":2,"is_corresponding":false},{"id":1276639,"name":"Martina Kojanová","orcid":"0000-0001-9890-9965","position":3,"is_corresponding":false},{"id":1276640,"name":"Miluše Kreidlová","orcid":"0000-0002-9472-6894","position":4,"is_corresponding":false},{"id":1276641,"name":"Filip Rob","orcid":"0000-0001-5257-205X","position":5,"is_corresponding":false},{"id":1277066,"name":"Vladimı́r Vašků","orcid":null,"position":6,"is_corresponding":false},{"id":1277067,"name":"Petra Mosio","orcid":null,"position":7,"is_corresponding":false},{"id":1277068,"name":"Radim Strnadel","orcid":null,"position":8,"is_corresponding":false},{"id":1277069,"name":"Olga Faustmannová","orcid":null,"position":9,"is_corresponding":false},{"id":1276642,"name":"Ivana Kuklová","orcid":"0000-0001-6917-8462","position":10,"is_corresponding":false},{"id":1276643,"name":"Monika Dvořáková Heroldová","orcid":"0000-0002-1373-464X","position":11,"is_corresponding":false},{"id":1277070,"name":"Hana Zákoucká","orcid":null,"position":12,"is_corresponding":false},{"id":486379,"name":"David Šmajs","orcid":"0000-0002-4176-3464","position":13,"is_corresponding":false},{"id":1277064,"name":"Eliška Vrbová","orcid":null,"position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-19T02:03:03.428151Z","pmid":"39075238","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}