{"doi":"10.1038/s41598-024-68433-4","title":"Increased levels of circulating cell-free DNA in COVID-19 patients with respiratory failure","abstract":"Cell-free DNA (cfDNA) is released from injured cells and aggravates inflammation. Patients with coronavirus disease (COVID-19) often develop pneumonia and respiratory failure, and require oxygen therapy (OT), including mechanical ventilation (MV). It remains unclear whether cfDNA predicts the risk of receiving OT or MV in COVID-19 patients. Therefore, we hypothesized that circulating cfDNA levels could reflect the severity of respiratory failure and determine a therapeutic approach for oxygenation in patients with COVID-19. We analyzed cfDNA levels in serum samples from 95 hospitalized patients with COVID-19 at Showa University Hospital (Tokyo, Japan). cfDNA levels were assessed by measuring the copy numbers of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) using quantitative real-time PCR (qPCR). Both cf-nDNA and cf-mtDNA levels were negatively correlated with adjusted SpO 2 for FiO 2 (SpO 2 /FiO 2 ratio). Elevated cf-nDNA and cf-mtDNA levels were associated with the requirement for OT or MV during patient admission. Multivariate logistic regression analysis revealed that cf-nDNA and cf-mtDNA levels were independent risk factors for OT and MV. These results suggest that both serum cf-nDNA and cf-mtDNA could serve as useful early biomarkers to indicate the necessity of OT or MV in patients with COVID-19.","journal":"Scientific Reports","year":2024,"id":433231,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1238965,"name":"Katsuki Wakayama","orcid":null,"position":1,"is_corresponding":false},{"id":1238485,"name":"Y Fukuda","orcid":"0000-0002-7087-1595","position":2,"is_corresponding":false},{"id":1238966,"name":"Shin Ohta","orcid":null,"position":3,"is_corresponding":false},{"id":379900,"name":"Tetsuya Homma","orcid":null,"position":4,"is_corresponding":false},{"id":1238967,"name":"Koichi Ando","orcid":null,"position":5,"is_corresponding":false},{"id":1238486,"name":"Yuji Nishihara","orcid":"0000-0003-4443-2377","position":6,"is_corresponding":false},{"id":1238487,"name":"Ryuichi Nakano","orcid":"0000-0003-2086-2591","position":7,"is_corresponding":false},{"id":401957,"name":"Jing Zhao","orcid":"0000-0001-5576-644X","position":8,"is_corresponding":false},{"id":1238488,"name":"Yuki Suzuki","orcid":"0000-0002-2509-8513","position":9,"is_corresponding":false},{"id":1238489,"name":"Yoji Kyotani","orcid":"0000-0002-7771-6889","position":10,"is_corresponding":false},{"id":1238490,"name":"Hisakazu Yano","orcid":"0000-0003-2085-7194","position":11,"is_corresponding":false},{"id":1238491,"name":"Kei Kasahara","orcid":"0000-0001-6431-7208","position":12,"is_corresponding":false},{"id":364892,"name":"Kuei‐Pin Chung","orcid":"0000-0003-1916-8068","position":13,"is_corresponding":false},{"id":1238492,"name":"Hironori Sagara","orcid":"0000-0003-2376-1606","position":14,"is_corresponding":false},{"id":1238968,"name":"Masanori Yoshizumi","orcid":null,"position":15,"is_corresponding":false},{"id":325026,"name":"Kiichi Nakahira","orcid":null,"position":16,"is_corresponding":false},{"id":1238964,"name":"Akihiko Tanaka","orcid":null,"position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-19T01:59:44.444627Z","pmid":"39075117","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}