{"doi":"10.1038/s41598-024-59104-5","title":"The development of lateral flow devices for urinary biomarkers to assess kidney health","abstract":"Serum creatinine levels are insensitive to real-time changes in kidney function or injury. There is a growing interest in assessing kidney injury by measuring biomarkers in body fluid. From our previous studies, we identified and reported three urinary biomarkers namely Uromodulin (UMOD), Osteopontin (OPN), and Interleukin-9 (IL-9) to be associated with kidney health. The availability of a rapid point-of-care test for these urinary biomarkers will potentially accelerate its applicability and accessibility. In this study, we aimed to develop novel lateral flow device (LFD) for UMOD, OPN and IL-9. We tested paired antibodies using Enzyme Linked Immunosorbent Assay wherein we observed functionality only for UMOD and OPN and not for IL-9. A conjugation buffer pH of 7.8 and 8.5 was found suitable at a detection antibody concentration of 15 µg/mL for LFD development. The developed LFDs were found to quantitatively measure UMOD standard (LLOD of 80,000 pg/mL) and OPN standard (LLOD of 8600 pg/mL) respectively. The LFD was also able to measure human urinary UMOD and OPN with a percent CV of 12.12 and 5.23 respectively.","journal":"Scientific Reports","year":2024,"id":453546,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":406754,"name":"Wassim Obeid","orcid":"0000-0002-6054-411X","position":1,"is_corresponding":false},{"id":1276854,"name":"Jeanine Hernandez","orcid":null,"position":2,"is_corresponding":false},{"id":416071,"name":"David Hu","orcid":"0000-0001-9306-4705","position":3,"is_corresponding":false},{"id":241231,"name":"Yumeng Wen","orcid":"0000-0002-1392-6369","position":4,"is_corresponding":false},{"id":258520,"name":"Dennis G. Moledina","orcid":"0000-0002-9537-9038","position":5,"is_corresponding":false},{"id":1276855,"name":"André Albert","orcid":null,"position":6,"is_corresponding":false},{"id":1276856,"name":"Anya Gregg","orcid":null,"position":7,"is_corresponding":false},{"id":1276429,"name":"Andrew Wheeler","orcid":"0000-0001-7480-8342","position":8,"is_corresponding":false},{"id":249664,"name":"Heather Thiessen‐Philbrook","orcid":"0000-0003-2067-0514","position":9,"is_corresponding":false},{"id":241229,"name":"Chirag R. Parikh","orcid":"0000-0001-9051-7385","position":10,"is_corresponding":false},{"id":1276853,"name":"Serena D Souza","orcid":null,"position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T02:03:03.428151Z","pmid":"38609491","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}