{"doi":"10.1038/s41598-024-55296-y","title":"ImmunoPET imaging of TIGIT in the glioma microenvironment","abstract":"Abstract Glioblastoma (GBM) is the most common primary malignant brain tumor. Currently, there are few effective treatment options for GBM beyond surgery and chemo-radiation, and even with these interventions, median patient survival remains poor. While immune checkpoint inhibitors (ICIs) have demonstrated therapeutic efficacy against non-central nervous system cancers, ICI trials for GBM have typically had poor outcomes. TIGIT is an immune checkpoint receptor that is expressed on activated T-cells and has a role in the suppression of T-cell and Natural Killer (NK) cell function. As TIGIT expression is reported as both prognostic and a biomarker for anti-TIGIT therapy, we constructed a molecular imaging agent, [ 89 Zr]Zr-DFO-anti-TIGIT ( 89 Zr-αTIGIT), to visualize TIGIT in preclinical GBM by immunoPET imaging. PET imaging and biodistribution analysis of 89 Zr-αTIGIT demonstrated uptake in the tumor microenvironment of GBM-bearing mice. Blocking antibody and irrelevant antibody tracer studies demonstrated specificity of 89 Zr-αTIGIT with significance at a late time point post-tracer injection. However, the magnitude of 89 Zr-αTIGIT uptake in tumor, relative to the IgG tracer was minimal. These findings highlight the features and limitations of using 89 Zr-αTIGIT to visualize TIGIT in the GBM microenvironment.","journal":"Scientific Reports","year":2024,"id":439863,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1064819,"name":"Ambika P. Jaswal","orcid":"0000-0002-1929-0082","position":1,"is_corresponding":false},{"id":397341,"name":"Stephen C. Frederico","orcid":"0000-0003-0232-9187","position":2,"is_corresponding":false},{"id":1064814,"name":"Michal Nisnboym","orcid":"0000-0003-0292-6344","position":3,"is_corresponding":false},{"id":1064818,"name":"Bo Li","orcid":"0000-0003-2397-4492","position":4,"is_corresponding":false},{"id":1064816,"name":"Zujian Xiong","orcid":"0000-0003-2943-1931","position":5,"is_corresponding":false},{"id":1064820,"name":"ReidAnn E. Sever","orcid":"0009-0007-7063-2600","position":6,"is_corresponding":false},{"id":642501,"name":"Chaim T. Sneiderman","orcid":"0009-0007-3726-6143","position":7,"is_corresponding":false},{"id":1251556,"name":"Mikayla Rodgers","orcid":null,"position":8,"is_corresponding":false},{"id":736116,"name":"Kathryn E. Day","orcid":"0009-0004-2279-0338","position":9,"is_corresponding":false},{"id":499184,"name":"Joseph D. Latoche","orcid":"0000-0002-4800-5623","position":10,"is_corresponding":false},{"id":272172,"name":"Lesley M. Foley","orcid":"0000-0001-7752-2139","position":11,"is_corresponding":false},{"id":272173,"name":"T. Kevin Hitchens","orcid":"0000-0001-6576-6742","position":12,"is_corresponding":false},{"id":415045,"name":"Robin Frederick","orcid":null,"position":13,"is_corresponding":false},{"id":373399,"name":"Ravi B. Patel","orcid":"0000-0002-8328-746X","position":14,"is_corresponding":false},{"id":446784,"name":"Costas G. Hadjipanayis","orcid":"0000-0001-8615-6644","position":15,"is_corresponding":false},{"id":240477,"name":"Itay Raphael","orcid":"0000-0002-7369-2752","position":16,"is_corresponding":false},{"id":107712,"name":"Jessie R. Nedrow","orcid":"0000-0002-3465-1296","position":17,"is_corresponding":false},{"id":783910,"name":"Wilson B. Edwards","orcid":"0000-0002-6070-8778","position":18,"is_corresponding":false},{"id":400563,"name":"Gary Kohanbash","orcid":"0000-0002-3953-8022","position":19,"is_corresponding":false},{"id":1064815,"name":"Sarah R. Vincze","orcid":"0009-0003-0735-6827","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T02:00:52.633010Z","pmid":"38438420","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}