{"doi":"10.1038/s41598-024-53841-3","title":"Cooperative control of a DNA origami force sensor","abstract":"Biomolecular systems are dependent on a complex interplay of forces. Modern force spectroscopy techniques provide means of interrogating these forces, but they are not optimized for studies in constrained environments as they require attachment to micron-scale probes such as beads or cantilevers. Nanomechanical devices are a promising alternative, but this requires versatile designs that can be tuned to respond to a wide range of forces. We investigate the properties of a nanoscale force sensitive DNA origami device which is highly customizable in geometry, functionalization, and mechanical properties. The device, referred to as the NanoDyn, has a binary (open or closed) response to an applied force by undergoing a reversible structural transition. The transition force is tuned with minor alterations of 1 to 3 DNA oligonucleotides and spans tens of picoNewtons (pN). The DNA oligonucleotide design parameters also strongly influence the efficiency of resetting the initial state, with higher stability devices (≳10 pN) resetting more reliably during repeated force-loading cycles. Finally, we show the opening force is tunable in real time by adding a single DNA oligonucleotide. These results establish the potential of the NanoDyn as a versatile force sensor and provide fundamental insights into how design parameters modulate mechanical and dynamic properties.","journal":"Scientific Reports","year":2024,"id":436225,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9485,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1172487,"name":"Hazen Hildebolt","orcid":null,"position":1,"is_corresponding":false},{"id":1172488,"name":"Michael Neuhoff","orcid":null,"position":2,"is_corresponding":false},{"id":802043,"name":"Peter E. Beshay","orcid":"0000-0003-2191-8219","position":3,"is_corresponding":false},{"id":424603,"name":"Jessica O. Winter","orcid":"0000-0002-5370-0137","position":4,"is_corresponding":false},{"id":288391,"name":"Carlos E. Castro","orcid":"0000-0001-7023-6105","position":5,"is_corresponding":false},{"id":333604,"name":"Ralf Bundschuh","orcid":"0000-0002-6699-8614","position":6,"is_corresponding":false},{"id":111546,"name":"Michael G. Poirier","orcid":"0000-0002-1563-5792","position":7,"is_corresponding":false},{"id":1172486,"name":"Ariel Robbins","orcid":null,"position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T02:00:12.706156Z","pmid":"38374280","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}