{"doi":"10.1038/s41598-021-97060-6","title":"A prelude to the proximity interaction mapping of CXXC5","abstract":"CXXC5 is a member of the zinc-finger CXXC family proteins that interact with unmodified CpG dinucleotides through a conserved ZF-CXXC domain. CXXC5 is involved in the modulation of gene expressions that lead to alterations in diverse cellular events. However, the underlying mechanism of CXXC5-modulated gene expressions remains unclear. Proteins perform their functions in a network of proteins whose identities and amounts change spatiotemporally in response to various stimuli in a lineage-specific manner. Since CXXC5 lacks an intrinsic transcription regulatory function or enzymatic activity but is a DNA binder, CXXC5 by interacting with proteins could act as a scaffold to establish a chromatin state restrictive or permissive for transcription. To initially address this, we utilized the proximity-dependent biotinylation approach. Proximity interaction partners of CXXC5 include DNA and chromatin modifiers, transcription factors/co-regulators, and RNA processors. Of these, CXXC5 through its CXXC domain interacted with EMD, MAZ, and MeCP2. Furthermore, an interplay between CXXC5 and MeCP2 was critical for a subset of CXXC5 target gene expressions. It appears that CXXC5 may act as a nucleation factor in modulating gene expressions. Providing a prelude for CXXC5 actions, our results could also contribute to a better understanding of CXXC5-mediated cellular processes in physiology and pathophysiology.","journal":"Scientific Reports","year":2021,"id":199350,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":775789,"name":"Gizem Turan","orcid":"0000-0002-5670-1135","position":1,"is_corresponding":false},{"id":775790,"name":"Çağla Ece Olgun","orcid":"0000-0002-5380-1851","position":2,"is_corresponding":false},{"id":776454,"name":"Gizem Kars","orcid":null,"position":3,"is_corresponding":false},{"id":775791,"name":"Burcu Karakaya","orcid":"0000-0002-4715-3093","position":4,"is_corresponding":false},{"id":775792,"name":"Kerim Yavuz","orcid":"0000-0003-4980-8804","position":5,"is_corresponding":false},{"id":775793,"name":"Öykü Deniz Demiralay","orcid":"0000-0003-4322-0305","position":6,"is_corresponding":false},{"id":732803,"name":"Tolga Can","orcid":"0000-0002-5532-0154","position":7,"is_corresponding":false},{"id":775794,"name":"Mesut Muyan","orcid":"0000-0003-3058-6228","position":8,"is_corresponding":false},{"id":775795,"name":"Pelin Yaşar","orcid":"0000-0003-3027-9842","position":9,"is_corresponding":false},{"id":691004,"name":"Gamze Ayaz","orcid":"0000-0002-1553-2558","position":0,"is_corresponding":true}],"reference_count":103,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:50:40.558460Z","pmid":"34475492","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}