{"doi":"10.1038/s41598-021-91793-0","title":"Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection.</jats:p>","journal":"Scientific Reports","year":2021,"id":20745,"datarank":0.9057807634426578,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.41706628273943536,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.41706628273943536,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":20,"citers_with_citation_signal":18,"citers_with_endowment":18,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136078,"name":"Hisham Ahmed Imad","orcid":null,"position":1,"is_corresponding":false},{"id":136079,"name":"Weerapong Phumratanaprapin","orcid":null,"position":2,"is_corresponding":false},{"id":136080,"name":"Juthamas Phadungsombat","orcid":null,"position":3,"is_corresponding":false},{"id":136081,"name":"Eiji Konishi","orcid":null,"position":4,"is_corresponding":false},{"id":136082,"name":"Tatsuo Shioda","orcid":null,"position":5,"is_corresponding":false},{"id":136077,"name":"Atsushi Yamanaka","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.258096538021482,"endowment":3.258096538021482,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34117329","pmcid":"PMC8196181","openalex_id":"https://openalex.org/W3169577540","authors":[],"funders":[{"funder_name":"Japan Agency for Medical Research and Development","grant_id":"JP18fm0108003","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia, I.P.","grant_id":"PTDC/CCI-BIO/29266/2017","title":"Deep Drug Discovery and Deployment"}],"total_grants":2,"fwci":3.3562,"citation_percentile":0.92178935,"influential_citations":0,"citation_trend":[{"year":2021,"count":4},{"year":2022,"count":7},{"year":2023,"count":4},{"year":2024,"count":2},{"year":2025,"count":5},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/s41598-021-91793-0.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/s41598-021-91793-0.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/s41598-021-91793-0","host_type":"publisher"},{"url":"https://doi.org/10.1038/s41598-021-91793-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34117329","host_type":"repository"},{"url":"https://doaj.org/article/fe6708c7f6214a3189d92335ffda592b","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8196181","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8196181","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8196181?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/2020.11.20.392357","host_type":""},{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/11/23/2020.11.20.392357.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.60692/9sxph-ak507","host_type":""},{"url":"https://dx.doi.org/10.60692/ergfm-95j08","host_type":""},{"url":"http://dx.doi.org/10.1038/s41598-021-91793-0","host_type":""},{"url":"https://dx.doi.org/10.1038/s41598-021-91793-0","host_type":""},{"url":"https://dx.doi.org/10.1101/2020.11.20.392357","host_type":""}],"fields_of_study":["Mosquito-borne diseases and control","Viral Infections and Vectors","Vector-borne infectious diseases","0301 basic medicine","03 medical and health sciences","0303 health sciences","Animals","Antibodies, Viral","Chlorocebus aethiops","Dengue","Dengue Virus","Humans","K562 Cells","Vero Cells","Viral Load","Viremia"],"mesh_terms":["Animals","Antibodies, Viral","Chlorocebus aethiops","Dengue","Dengue Virus","Humans","Vero Cells","Viremia","Viral Load","K562 Cells"],"keywords":["Viremia","Dengue fever","Dengue virus","Virology","Antibody","Antibody-dependent enhancement","Virus","Titer","Neutralizing antibody","Viral load","Immunology","Biology","In vivo","Viral disease","Antibody titer","Medicine","Science","FOS: Health sciences","Dengue vaccine","Antibodies, Viral","Article","Dengue","Chlorocebus aethiops","Health Sciences","Animals","Humans","Global Impact of Arboviral Diseases","Vero Cells","Immunology and Microbiology","FOS: Clinical medicine","Q","R","Public Health, Environmental and Occupational Health","Life Sciences","Infectious Diseases","Tick-Borne Diseases and Pathogens Transmission","Parasitology","K562 Cells","Viral Hemorrhagic Fevers and Zoonotic Infections","Biotechnology"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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