{"doi":"10.1038/s41598-021-02869-w","title":"Myeloid-associated differentiation marker is a novel SP-A-associated transmembrane protein whose expression on airway epithelial cells correlates with asthma severity","abstract":"Surfactant protein A (SP-A) is well-known for its protective role in pulmonary immunity. Previous studies from our group have shown that SP-A mediates eosinophil activities, including degranulation and apoptosis. In order to identify potential binding partners on eosinophils for SP-A, eosinophil lysates were subjected to SP-A pull-down and tandem mass spectrometry (MS/MS) analysis. We identified one membrane-bound protein, myeloid-associated differentiation marker (MYADM), as a candidate SP-A binding partner. Blocking MYADM on mouse and human eosinophils ex vivo prevented SP-A from inducing apoptosis; blocking MYADM in vivo led to increased persistence of eosinophilia and airway hyper-responsiveness in an ovalbumin (OVA) allergy model and increased airways resistance and mucus production in a house dust mite (HDM) asthma model. Examination of a subset of participants in the Severe Asthma Research Program (SARP) cohort revealed a significant association between epithelial expression of MYADM in asthma patients and parameters of airway inflammation, including: peripheral blood eosinophilia, exhaled nitric oxide (FeNO) and the number of exacerbations in the past 12 months. Taken together, our studies provide the first evidence of MYADM as a novel SP-A-associated protein that is necessary for SP-A to induce eosinophil apoptosis and we bring to light the potential importance of this previously unrecognized transmembrane protein in patients with asthma.","journal":"Scientific Reports","year":2021,"id":199546,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9693,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":467723,"name":"Paul R. Langlais","orcid":"0000-0003-1929-737X","position":1,"is_corresponding":false},{"id":488554,"name":"Natalie K. Barker","orcid":"0000-0001-9248-2710","position":2,"is_corresponding":false},{"id":334210,"name":"Kenneth J. Addison","orcid":"0000-0002-8964-9628","position":3,"is_corresponding":false},{"id":776063,"name":"Sasipa Tanyaratsrisakul","orcid":"0000-0002-2188-5990","position":4,"is_corresponding":false},{"id":398416,"name":"Scott Boitano","orcid":null,"position":5,"is_corresponding":false},{"id":250080,"name":"Stephanie A. Christenson","orcid":"0000-0003-1550-2815","position":6,"is_corresponding":false},{"id":232046,"name":"Monica Kraft","orcid":"0000-0003-2626-2183","position":7,"is_corresponding":false},{"id":24823,"name":"Deborah A. Meyers","orcid":"0000-0001-5399-8423","position":8,"is_corresponding":false},{"id":250079,"name":"Eugene R. Bleecker","orcid":"0000-0002-4767-3494","position":9,"is_corresponding":false},{"id":268133,"name":"Xingnan Li","orcid":"0000-0002-1208-8658","position":10,"is_corresponding":false},{"id":70361,"name":"Julie G. Ledford","orcid":"0000-0001-7977-4575","position":11,"is_corresponding":false},{"id":334209,"name":"Alane Blythe C. Dy","orcid":"0000-0002-9899-6090","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:50:40.558460Z","pmid":"34862427","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}