{"doi":"10.1038/s41598-019-47660-0","title":"Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission","abstract":"<jats:title>Abstract</jats:title><jats:p>The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-like fluorophore still remains elusive. Here we present theoretical calculations to predict how hydrogen bonding could influence the excited states of the GFP-like fluorophores. These studies provide both a new perspective for understanding the photophysical properties of GFP as well as a solid basis for the rational design of GFP-based fluorophores.</jats:p>","journal":"Scientific 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Fluorescence Microscopy Techniques","Photoreceptor and optogenetics research","Advanced Biosensing Techniques and Applications","Medicine","Chemistry","Materials Science","Computational Chemistry","Fluorescence","Fluorescent Dyes","Green Fluorescent Proteins","Hydrogen Bonding","Models, Chemical","Spectrometry, Fluorescence","Water"],"mesh_terms":["Computational Chemistry","Fluorescence","Fluorescent Dyes","Hydrogen Bonding","Models, Chemical","Spectrometry, Fluorescence","Water","Green Fluorescent Proteins"],"keywords":["Fluorophore","Green fluorescent protein","Chromophore","Fluorescence","Hydrogen bond","Photochemistry","Molecule","Excited state","Chemistry","Biophysics","Biochemistry","Biology","Physics","Optics","Atomic 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