{"doi":"10.1038/s41588-024-01902-8","title":"Antagonism between H3K27me3 and genome–lamina association drives atypical spatial genome organization in the totipotent embryo","abstract":null,"journal":"Nature Genetics","year":2024,"id":620122,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1600670,"name":"Franka J. Rang","orcid":null,"position":1,"is_corresponding":false},{"id":1600671,"name":"Yumiko K. Kawamura","orcid":null,"position":2,"is_corresponding":false},{"id":1600672,"name":"Carla Kroon-Veenboer","orcid":null,"position":3,"is_corresponding":false},{"id":1600673,"name":"Jeroen Korving","orcid":null,"position":4,"is_corresponding":false},{"id":1600674,"name":"Femke C. Groenveld","orcid":null,"position":5,"is_corresponding":false},{"id":1600675,"name":"Ramada E. van Beek","orcid":null,"position":6,"is_corresponding":false},{"id":1600676,"name":"Silke J. A. Lochs","orcid":"0000-0001-8066-2984","position":7,"is_corresponding":false},{"id":1600677,"name":"Ellen Boele","orcid":null,"position":8,"is_corresponding":false},{"id":1600678,"name":"Antoine H. M. F. Peters","orcid":"0000-0002-0311-1887","position":9,"is_corresponding":false},{"id":39699,"name":"Jop Kind","orcid":"0000-0001-7538-2638","position":10,"is_corresponding":false},{"id":1600669,"name":"Isabel Guerreiro","orcid":"0000-0003-1390-3535","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Antagonism between H3K27me3 and genome–lamina association drives atypical spatial genome organization in the totipotent embryo","abstract":"In mammals, early embryonic development exhibits highly unusual spatial positioning of genomic regions at the nuclear lamina, but the mechanisms underpinning this atypical genome organization remain elusive. Here, we generated single-cell profiles of lamina-associated domains (LADs) coupled with transcriptomics, which revealed a striking overlap between preimplantation-specific LAD dissociation and noncanonical broad domains of H3K27me3. Loss of H3K27me3 resulted in a restoration of canonical LAD profiles, suggesting an antagonistic relationship between lamina association and H3K27me3. Tethering of H3K27me3 to the nuclear periphery showed that the resultant relocalization is partially dependent on the underlying DNA sequence. Collectively, our results suggest that the atypical organization of LADs in early developmental stages is the result of a tug-of-war between intrinsic affinity for the nuclear lamina and H3K27me3, constrained by the available space at the nuclear periphery. This study provides detailed insight into the molecular mechanisms regulating nuclear organization during early mammalian development. Single-cell profiling of lamina-associated domains (LADs) during early mouse development reveals an overlap between preimplantation-specific LAD dissociation and noncanonical broad H3K27me3 domains. Loss of H3K27me3 restores canonical LAD profiles.","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39284976","pmcid":"PMC11525175","openalex_id":"https://openalex.org/W4402580936","authors":[],"funders":[{"funder_name":"Dutch Research Council (NWO)","grant_id":"VI.Veni.202.073","title":null},{"funder_name":"Dutch Research Council (NWO)","grant_id":"184.034.019","title":"Netherlands X-omics Initiative"},{"funder_name":"European Research Council","grant_id":"101002885","title":"Learning from the past: linking ancestral epigenetic states to current cellular fates with single-cell multi-omic approaches"},{"funder_name":"European Research Council","grant_id":"695288","title":"Transcriptional and Epigenetic Regulation of Totipotency in Mouse Early Embryos."},{"funder_name":"European Research Council","grant_id":"678423","title":"Single-cell epigenomics: quantifying epigenetic changes in individual cells using DamID"}],"total_grants":5,"fwci":3.557,"citation_percentile":0.9407455,"influential_citations":0,"citation_trend":[{"year":2024,"count":3},{"year":2025,"count":13},{"year":2026,"count":6}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1038/s41588-024-01902-8","host_type":"journal"},{"url":"https://doi.org/10.1038/s41588-024-01902-8","host_type":"publisher"},{"url":"https://www.nature.com/articles/s41588-024-01902-8.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/s41588-024-01902-8","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39284976","host_type":"repository"},{"url":"https://pure.knaw.nl/portal/en/publications/15abee90-9f85-45af-b70b-7012a8dad3fa","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11525175","host_type":"repository"},{"url":"https://dspace.library.uu.nl/handle/1874/458275","host_type":"repository"},{"url":"https://hdl.handle.net/20.500.11755/15abee90-9f85-45af-b70b-7012a8dad3fa","host_type":"repository"},{"url":"https://hdl.handle.net/2066/312037","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11525175","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11525175?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1038/s41588-024-01902-8","host_type":""},{"url":"https://hdl.handle.net/https://repository.ubn.ru.nl/handle/2066/312037","host_type":""},{"url":"https://repository.ubn.ru.nl//bitstream/handle/2066/312037/312037.pdf","host_type":""}],"fields_of_study":["RNA Research and Splicing","Genomics and Chromatin Dynamics","Nuclear Structure and Function"],"mesh_terms":["Animals","Embryo, Mammalian","Female","Histones","Genome","Gene Expression Regulation, Developmental","Nuclear Lamina","Embryonic Development","Mice","Single-Cell Analysis"],"keywords":["Biology","Nuclear lamina","Totipotent","Genomic organization","Genome","Spatial organization","Lamin","Evolutionary biology","Genetics","Embryo","Chromatin","Cell biology","Embryonic stem cell","Computational biology","Gene","Transcription factor","Nuclear protein","Nuclear Lamina/metabolism","Single-Cell Analysis/methods","Histones/metabolism","Embryonic Development","Gene Expression Regulation, Developmental","Mammalian/metabolism","Embryo, Mammalian","Article","Histones","Mice","Gene Expression Regulation","Genome/genetics","Embryonic Development/genetics","Animals","Developmental","Female","Single-Cell Analysis","Molecular Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"geo"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T10:01:30.305817Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}