{"doi":"10.1038/s41564-024-01915-3","title":"KorB switching from DNA-sliding clamp to repressor mediates long-range gene silencing in a multi-drug resistance plasmid","abstract":"Examples of long-range gene regulation in bacteria are rare and generally thought to involve DNA looping. Here, using a combination of biophysical approaches including X-ray crystallography and single-molecule analysis for the KorB-KorA system in Escherichia coli, we show that long-range gene silencing on the plasmid RK2, a source of multi-drug resistance across diverse Gram-negative bacteria, is achieved cooperatively by a DNA-sliding clamp, KorB, and a clamp-locking protein, KorA. We show that KorB is a CTPase clamp that can entrap and slide along DNA to reach distal target promoters up to 1.5 kb away. We resolved the tripartite crystal structure of a KorB-KorA-DNA co-complex, revealing that KorA latches KorB into a closed clamp state. DNA-bound KorA thus stimulates repression by stalling KorB sliding at target promoters to occlude RNA polymerase holoenzymes. Together, our findings explain the mechanistic basis for KorB role switching from a DNA-sliding clamp to a co-repressor and provide an alternative mechanism for long-range regulation of gene expression in bacteria.","journal":"Nature Microbiology","year":2025,"id":511685,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1135680,"name":"Francisco de Asís Balaguer","orcid":"0000-0002-6393-2863","position":1,"is_corresponding":false},{"id":893169,"name":"Joshua Chandanani","orcid":"0000-0003-2671-2412","position":2,"is_corresponding":false},{"id":924041,"name":"Christopher M. Thomas","orcid":"0000-0002-2810-0455","position":3,"is_corresponding":false},{"id":678695,"name":"Clara Aicart-Ramos","orcid":"0000-0002-1114-4259","position":4,"is_corresponding":false},{"id":1327674,"name":"Sophia Burick","orcid":"0009-0009-1160-2414","position":5,"is_corresponding":false},{"id":471220,"name":"Paul Dominic B. Olinares","orcid":"0000-0002-3429-6618","position":6,"is_corresponding":false},{"id":1328119,"name":"Giulia Gobbato","orcid":null,"position":7,"is_corresponding":false},{"id":1327675,"name":"Julia E. A. Mundy","orcid":"0009-0006-6232-7254","position":8,"is_corresponding":false},{"id":51698,"name":"Brian T. Chait","orcid":"0000-0003-3524-557X","position":9,"is_corresponding":false},{"id":1327676,"name":"David M. Lawson","orcid":"0000-0002-7637-4303","position":10,"is_corresponding":false},{"id":258065,"name":"Seth A. Darst","orcid":"0000-0002-8241-3153","position":11,"is_corresponding":false},{"id":461537,"name":"Elizabeth A. Campbell","orcid":"0000-0002-1332-128X","position":12,"is_corresponding":false},{"id":678699,"name":"Fernando Moreno‐Herrero","orcid":"0000-0003-4083-1709","position":13,"is_corresponding":false},{"id":887088,"name":"Tung B. K. Le","orcid":"0000-0003-4764-8851","position":14,"is_corresponding":false},{"id":1327673,"name":"Thomas C. McLean","orcid":"0000-0002-6467-4974","position":0,"is_corresponding":true}],"reference_count":98,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:47:55.918021Z","pmid":"39849085","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}