{"doi":"10.1038/s41564-019-0657-5","title":"Diffusion and capture permits dynamic coupling between treadmilling FtsZ filaments and cell division proteins","abstract":null,"journal":"Nature Microbiology","year":2020,"id":672008,"datarank":0.6476232170304466,"base_score":4.31748811353631,"endowment":4.31748811353631,"self_citation_contribution":0.6476232170304466,"citation_network_contribution":0.0,"self_endowment_contribution":0.6476232170304466,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1755673,"name":"Philipp Radler","orcid":null,"position":1,"is_corresponding":false},{"id":1755674,"name":"Víctor M. Hernández-Rocamora","orcid":null,"position":2,"is_corresponding":false},{"id":540787,"name":"Carlos Alfonso","orcid":"0000-0001-7165-4800","position":3,"is_corresponding":false},{"id":1755675,"name":"Mar López-Pelegrín","orcid":null,"position":4,"is_corresponding":false},{"id":565044,"name":"Germán Rivas","orcid":"0000-0003-3450-7478","position":5,"is_corresponding":false},{"id":308355,"name":"Waldemar Vollmer","orcid":"0000-0003-0408-8567","position":6,"is_corresponding":false},{"id":776227,"name":"Martin Loose","orcid":"0000-0001-7309-9724","position":7,"is_corresponding":false},{"id":1755672,"name":"Natalia Baranova","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Diffusion and capture permits dynamic coupling between treadmilling FtsZ filaments and cell division proteins","abstract":"Most bacteria accomplish cell division with the help of a dynamic protein complex called the divisome, which spans the cell envelope in the plane of division. Assembly and activation of this machinery are coordinated by the tubulin-related GTPase FtsZ, which was found to form treadmilling filaments on supported bilayers in vitro<sup>1</sup>, as well as in live cells, in which filaments circle around the cell division site<sup>2,3</sup>. Treadmilling of FtsZ is thought to actively move proteins around the division septum, thereby distributing peptidoglycan synthesis and coordinating the inward growth of the septum to form the new poles of the daughter cells<sup>4</sup>. However, the molecular mechanisms underlying this function are largely unknown. Here, to study how FtsZ polymerization dynamics are coupled to downstream proteins, we reconstituted part of the bacterial cell division machinery using its purified components FtsZ, FtsA and truncated transmembrane proteins essential for cell division. We found that the membrane-bound cytosolic peptides of FtsN and FtsQ co-migrated with treadmilling FtsZ-FtsA filaments, but despite their directed collective behaviour, individual peptides showed random motion and transient confinement. Our work suggests that divisome proteins follow treadmilling FtsZ filaments by a diffusion-and-capture mechanism, which can give rise to a moving zone of signalling activity at the division site.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31959972","pmcid":"PMC7048620","openalex_id":null,"authors":[],"funders":[{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/R017409/1","title":"Membrane steps in bacterial cell wall synthesis"},{"funder_name":"Wellcome Trust","grant_id":"101824/Z/13/Z","title":null},{"funder_name":"European Research Council","grant_id":"679239","title":"Self-Organization of the Bacterial Cell"},{"funder_name":"Wellcome Trust","grant_id":"101824","title":"Bacterial Cell Wall Synthesis and Degradation."},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":5,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"Springer Nature TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7048620","host_type":"repository"},{"url":"https://www.nature.com/articles/s41564-019-0657-5.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/s41564-019-0657-5","host_type":"publisher"},{"url":"https://europepmc.org/articles/PMC7048620","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7048620?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1038/s41564-019-0657-5","host_type":""},{"url":"https://europepmc.org/articles/pmc7048620?pdf=render","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/31959972","host_type":""},{"url":"http://dx.doi.org/10.1038/s41564-019-0657-5","host_type":""},{"url":"http://hdl.handle.net/10261/200009","host_type":""},{"url":"https://api.library.uq.edu.au/view/UQ:d9e80d5","host_type":""},{"url":"https://espace.library.uq.edu.au/view/UQ:d9e80d5","host_type":""},{"url":"https://dx.doi.org/10.1038/s41564-019-0657-5","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/s41564-019-0657-5","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Cell Wall","Cytoskeleton","Escherichia coli","GTP Phosphohydrolases","Bacterial Proteins","Escherichia coli Proteins","Cytoskeletal Proteins","Membrane Proteins","Cell Division","Diffusion"],"keywords":["Sedimentation-Velocity","Activation","Bacterial physiology","Article","GTP Phosphohydrolases","Diffusion","Single-molecule biophysics","Bacterial Proteins","Cell Wall","Membrane-Proteins","Escherichia coli","Subdomain","Peptidoglycan Synthesis","Divisome","Synthetic biology","Cytoskeleton","Cytokinesis","Escherichia coli Proteins","Membrane Proteins","Interacts","Cytoskeletal proteins","Localization","Recruitment","Ultracentrifugation","Cell Division"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"},{"name":"pdb"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T05:51:47.469793Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}