{"doi":"10.1038/s41541-024-01044-2","title":"A functional enzymatic assay as potential readout for a clinical trial of a schistosomiasis vaccine","abstract":"An estimated 200 million people are currently infected with schistosomiasis and an additional 800 million reside in high transmission-risk areas in 78 endemic countries. In this report we describe a functional enzymatic assay based on the core calpain antigen (Sm-p80) of the schistosomiasis vaccine, SchistoShield®. A 44 kDa soluble variant of the core Sm-p80 antigen (B7), was assessed for its enzymatic activity using a fluorescent synthetic substrate. Inhibition of the B7 enzymatic activity by Sm-p80-specific antibodies obtained from pre-clinical trials in rodents, non-human primates as well as from participants of the human clinical trials was measured. The B7 enzyme activity followed a Michaelis-Menten-like kinetic behavior. Statistically significant inhibition of the B7 activity was observed by Sm-p80-specific antibodies produced by immunized mice, non-human primates and humans. This quantitative serological assay could be of value in assessing the effectiveness of the SchistoShield® vaccine in human trials in Africa.","journal":"npj Vaccines","year":2025,"id":539013,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1426206,"name":"Mumtaz Yaseen Balkhi","orcid":null,"position":1,"is_corresponding":false},{"id":1425811,"name":"Yasuko Ono","orcid":"0000-0001-9588-0087","position":2,"is_corresponding":false},{"id":951804,"name":"Jenn Davis","orcid":null,"position":3,"is_corresponding":false},{"id":1426207,"name":"Naoko Doi","orcid":null,"position":4,"is_corresponding":false},{"id":1426208,"name":"Aryandra Arya","orcid":null,"position":5,"is_corresponding":false},{"id":490051,"name":"Jiho Kim","orcid":"0000-0001-5301-8130","position":6,"is_corresponding":false},{"id":1425812,"name":"Aravindan Kalyanasundaram","orcid":"0000-0001-6301-4328","position":7,"is_corresponding":false},{"id":1426209,"name":"Sourav Nandy","orcid":null,"position":8,"is_corresponding":false},{"id":1426210,"name":"Njariharinjakamampionona Rakotozandrindrainy","orcid":null,"position":9,"is_corresponding":false},{"id":530550,"name":"Bart L. Staker","orcid":"0000-0001-9570-5086","position":10,"is_corresponding":false},{"id":570826,"name":"Justin K. Craig","orcid":"0000-0001-5275-438X","position":11,"is_corresponding":false},{"id":670046,"name":"Raphaël Rakotozandrindrainy","orcid":null,"position":12,"is_corresponding":false},{"id":73234,"name":"Birkneh Tilahun Tadesse","orcid":"0000-0003-4005-8605","position":13,"is_corresponding":false},{"id":491677,"name":"Florian Marks","orcid":"0000-0002-6043-7170","position":14,"is_corresponding":false},{"id":218071,"name":"Lisa A. Jackson","orcid":"0000-0002-1785-0218","position":15,"is_corresponding":false},{"id":238503,"name":"Darrick Carter","orcid":"0000-0001-5901-3402","position":16,"is_corresponding":false},{"id":492885,"name":"Sean A. Gray","orcid":null,"position":17,"is_corresponding":false},{"id":491678,"name":"Afzal A. Siddiqui","orcid":"0000-0002-1217-4433","position":18,"is_corresponding":false},{"id":1425810,"name":"Desalegn W. Kifle","orcid":"0000-0002-0099-3415","position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T02:52:25.799399Z","pmid":"40082479","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}