{"doi":"10.1038/s41467-024-53745-w","title":"Open architecture of archaea MCM and dsDNA complexes resolved using monodispersed streptavidin affinity CryoEM","abstract":"The cryo-electron microscopy (cryoEM) method has enabled high-resolution structure determination of numerous biomolecules and complexes. Nevertheless, cryoEM sample preparation of challenging proteins and complexes, especially those with low abundance or with preferential orientation, remains a major hurdle. We developed an affinity-grid method employing monodispersed single particle streptavidin on a lipid monolayer to enhance particle absorption on the grid surface and alleviate sample exposure to the air-water interface. Using this approach, we successfully enriched the Thermococcus kodakarensis mini-chromosome maintenance complex 3 (MCM3) on cryoEM grids through biotinylation and resolved its structure. We further utilized this affinity method to tether the biotin-tagged dsDNA to selectively enrich a stable MCM3-ATP-dsDNA complex for cryoEM structure determination. Intriguingly, both MCM3 apo and dsDNA bound structures exhibit left-handed open spiral conformations, distinct from other reported MCM structures. The large open gate is sufficient to accommodate a dsDNA which could potentially be melted. The value of mspSA affinity method was further demonstrated by mitigating the issue of preferential angular distribution of HIV-1 capsid protein hexamer and RNA polymerase II elongation complex from Saccharomyces cerevisiae.","journal":"Nature Communications","year":2024,"id":456851,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1281696,"name":"Gangshun Yi","orcid":"0009-0004-6509-4893","position":1,"is_corresponding":false},{"id":1281697,"name":"Mingda Ye","orcid":"0000-0001-6324-4238","position":2,"is_corresponding":false},{"id":1021266,"name":"Craig MacGregor-Chatwin","orcid":"0000-0002-3995-3373","position":3,"is_corresponding":false},{"id":555773,"name":"Yuewen Sheng","orcid":"0000-0003-3067-9520","position":4,"is_corresponding":false},{"id":1281698,"name":"Ying Lü","orcid":"0000-0002-8421-7228","position":5,"is_corresponding":false},{"id":1226435,"name":"Ming Li","orcid":"0000-0002-5328-5826","position":6,"is_corresponding":false},{"id":817892,"name":"Qingrong Li","orcid":"0000-0002-8583-3620","position":7,"is_corresponding":false},{"id":498750,"name":"Dong Wang","orcid":"0000-0002-2829-1546","position":8,"is_corresponding":false},{"id":695230,"name":"Robert J.C. Gilbert","orcid":"0000-0001-9336-5604","position":9,"is_corresponding":false},{"id":245753,"name":"Peijun Zhang","orcid":"0000-0003-1803-691X","position":10,"is_corresponding":false},{"id":1281695,"name":"Jianbing Ma","orcid":"0000-0002-1665-1428","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-19T02:03:32.516678Z","pmid":"39604363","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}