{"doi":"10.1038/s41467-024-48929-3","title":"Direct RNA sequencing of astronaut blood reveals spaceflight-associated m6A increases and hematopoietic transcriptional responses","abstract":"Abstract The advent of civilian spaceflight challenges scientists to precisely describe the effects of spaceflight on human physiology, particularly at the molecular and cellular level. Newer, nanopore-based sequencing technologies can quantitatively map changes in chemical structure and expression at single molecule resolution across entire isoforms. We perform long-read, direct RNA nanopore sequencing, as well as Ultima high-coverage RNA-sequencing, of whole blood sampled longitudinally from four SpaceX Inspiration4 astronauts at seven timepoints, spanning pre-flight, day of return, and post-flight recovery. We report key genetic pathways, including changes in erythrocyte regulation, stress induction, and immune changes affected by spaceflight. We also present the first m 6 A methylation profiles for a human space mission, suggesting a significant spike in m 6 A levels immediately post-flight. These data and results represent the first longitudinal long-read RNA profiles and RNA modification maps for each gene for astronauts, improving our understanding of the human transcriptome’s dynamic response to spaceflight.","journal":"Nature Communications","year":2024,"id":421945,"datarank":0.6584113798579807,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.15331700536000945,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.15331700536000945,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":16,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9069,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":980565,"name":"Theodore M. Nelson","orcid":"0000-0002-8600-0444","position":1,"is_corresponding":false},{"id":1163544,"name":"Eliah Overbey","orcid":"0000-0002-2866-8294","position":2,"is_corresponding":false},{"id":787271,"name":"Nadia Houerbi","orcid":null,"position":3,"is_corresponding":false},{"id":1163545,"name":"JangKeun Kim","orcid":"0000-0002-8733-9925","position":4,"is_corresponding":false},{"id":1167078,"name":"Deena Najjar","orcid":"0009-0009-7950-2866","position":5,"is_corresponding":false},{"id":1113040,"name":"Namita Damle","orcid":null,"position":6,"is_corresponding":false},{"id":619413,"name":"Evan E. Afshin","orcid":null,"position":7,"is_corresponding":false},{"id":550379,"name":"Krista Ryon","orcid":"0000-0001-9440-2729","position":8,"is_corresponding":false},{"id":353586,"name":"Jean Thierry‐Mieg","orcid":"0000-0002-0396-6789","position":9,"is_corresponding":false},{"id":353585,"name":"Danielle Thierry‐Mieg","orcid":"0000-0001-9568-4211","position":10,"is_corresponding":false},{"id":237422,"name":"Ari Melnick","orcid":"0000-0002-8074-2287","position":11,"is_corresponding":false},{"id":1167345,"name":"Jaime Mateus","orcid":null,"position":12,"is_corresponding":false},{"id":36370,"name":"Christopher E. Mason","orcid":"0000-0002-1850-1642","position":13,"is_corresponding":false},{"id":324399,"name":"Kirill Grigorev","orcid":"0000-0003-3628-0123","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:57:41.370244Z","pmid":"38862496","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}