{"doi":"10.1038/s41467-024-48205-4","title":"The Shigella kinase effector OspG modulates host ubiquitin signaling to escape septin-cage entrapment","abstract":"Shigella flexneri is a Gram-negative bacterium causing severe bloody dysentery. Its pathogenesis is largely dictated by a plasmid-encoded type III secretion system (T3SS) and its associated effectors. Among these, the effector OspG has been shown to bind to the ubiquitin conjugation machinery (E2~Ub) to activate its kinase activity. However, the cellular targets of OspG remain elusive despite years of extensive efforts. Here we show by unbiased phosphoproteomics that a major target of OspG is CAND1, a regulatory protein controlling the assembly of cullin-RING ubiquitin ligases (CRLs). CAND1 phosphorylation weakens its interaction with cullins, which is expected to impact a large panel of CRL E3s. Indeed, global ubiquitome profiling reveals marked changes in the ubiquitination landscape when OspG is introduced. Notably, OspG promotes ubiquitination of a class of cytoskeletal proteins called septins, thereby inhibiting formation of cage-like structures encircling cytosolic bacteria. Overall, we demonstrate that pathogens have evolved an elaborate strategy to modulate host ubiquitin signaling to evade septin-cage entrapment.","journal":"Nature Communications","year":2024,"id":432209,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395229,"name":"Jiaqi Fu","orcid":"0000-0003-0081-6133","position":1,"is_corresponding":false},{"id":1236784,"name":"Qinxin Zhang","orcid":"0000-0003-3819-5504","position":2,"is_corresponding":false},{"id":678736,"name":"Chuang Li","orcid":"0000-0002-0271-1397","position":3,"is_corresponding":false},{"id":1215949,"name":"Yan‐Bo Zhao","orcid":"0000-0002-4229-1737","position":4,"is_corresponding":false},{"id":1236785,"name":"Zhiheng Tang","orcid":"0009-0000-1945-2072","position":5,"is_corresponding":false},{"id":1236786,"name":"Yi Yuan","orcid":"0000-0003-3914-2447","position":6,"is_corresponding":false},{"id":232122,"name":"Ying Wang","orcid":"0000-0001-7808-6279","position":7,"is_corresponding":false},{"id":1236787,"name":"Yan Zhou","orcid":"0000-0001-9352-7108","position":8,"is_corresponding":false},{"id":1237264,"name":"Peter S. Brzoic","orcid":null,"position":9,"is_corresponding":false},{"id":6649,"name":"Ning Zheng","orcid":"0000-0002-1039-1581","position":10,"is_corresponding":false},{"id":395236,"name":"Songying Ouyang","orcid":"0000-0002-1120-1524","position":11,"is_corresponding":false},{"id":395235,"name":"Zhao‐Qing Luo","orcid":"0000-0001-8890-6621","position":12,"is_corresponding":false},{"id":829558,"name":"Xiaoyun Liu","orcid":"0000-0001-7083-5263","position":13,"is_corresponding":false},{"id":1215951,"name":"Wei Xian","orcid":"0000-0002-3683-7983","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T01:59:35.267142Z","pmid":"38719850","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}