{"doi":"10.1038/nsmb1055","title":"Intermediates revealed in the kinetic mechanism for DNA unwinding by a monomeric helicase","abstract":null,"journal":"Nature Structural & Molecular Biology","year":2006,"id":671642,"datarank":1.9779206875863373,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":1.3911172367721154,"self_endowment_contribution":0.586803450814222,"citer_contribution":1.3911172367721154,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":31,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1754712,"name":"Kevin D Raney","orcid":null,"position":1,"is_corresponding":false},{"id":1754710,"name":"Robert L Eoff","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Intermediates revealed in the kinetic mechanism for DNA unwinding by a monomeric helicase","abstract":"Helicases unwind dsDNA during replication, repair and recombination in an ATP-dependent reaction. The mechanism for helicase activity can be studied using oligonucleotide substrates to measure formation of single-stranded (ss) DNA from double-stranded (ds) DNA. This assay provides an 'all-or-nothing' readout because partially unwound intermediates are not detected. We have determined conditions under which an intermediate in the reaction cycle of Dda helicase can be detected by trapping a partially unwound substrate. The appearance of this intermediate supports a model in which each ssDNA product interacts with the helicase after unwinding has occurred. Kinetic analysis indicates that the intermediate appears during a slow step in the reaction cycle that is flanked by faster steps for unwinding. These observations demonstrate a complex mechanism containing nonuniform steps for a monomeric helicase. The potential biological significance of such a mechanism is discussed.","is_dataset_classified":null,"base_score":3.912023005428146,"endowment":3.912023005428146,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16474403","pmcid":null,"openalex_id":"https://openalex.org/W1986881948","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM59400","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"P20 RR15569","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P20RR015569-10","title":"Center for Protein Structure and Function"},{"funder_name":"National Institutes of Health","grant_id":"2R01GM059400-06","title":"DNA Unwinding and Translocation by Helicases"}],"total_grants":4,"fwci":1.6535,"citation_percentile":0.81572999,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":3},{"year":2014,"count":5},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"http://www.nature.com/articles/nsmb1055.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nsmb1055","host_type":"publisher"},{"url":"https://doi.org/10.1038/nsmb1055","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16474403","host_type":"repository"},{"url":"https://dx.doi.org/10.1038/nsmb1055","host_type":""}],"fields_of_study":["DNA and Nucleic Acid Chemistry","DNA Repair Mechanisms","Bacterial Genetics and Biotechnology","0301 basic medicine","0303 health sciences","03 medical and health sciences","Base Pairing","Base Sequence","DNA","DNA Helicases","Kinetics","Least-Squares Analysis","Viral Proteins"],"mesh_terms":["Base Sequence","DNA","DNA Helicases","Kinetics","Viral Proteins","Least-Squares Analysis","Base Pairing"],"keywords":["Helicase","DNA","Homologous recombination","Biophysics","Oligonucleotide","Chemistry","DNA replication","Monomer","Biology","Biochemistry","RNA","Gene","Kinetics","Viral Proteins","Base Sequence","DNA Helicases","Least-Squares Analysis","Base Pairing"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T03:44:34.317000Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}