{"doi":"10.1038/nsmb.2832","title":"Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution","abstract":null,"journal":"Nature Structural & Molecular Biology","year":2014,"id":606564,"datarank":0.6535063240034389,"base_score":4.356708826689592,"endowment":4.356708826689592,"self_citation_contribution":0.6535063240034389,"citation_network_contribution":0.0,"self_endowment_contribution":0.6535063240034389,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":77,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1557214,"name":"Elizabeth C Curran","orcid":null,"position":1,"is_corresponding":false},{"id":1557215,"name":"Hong Hong Liao","orcid":null,"position":2,"is_corresponding":false},{"id":1557216,"name":"Kristie L Andrews","orcid":null,"position":3,"is_corresponding":false},{"id":1557217,"name":"Christine N Treba","orcid":null,"position":4,"is_corresponding":false},{"id":1506488,"name":"Samuel E Butcher","orcid":null,"position":5,"is_corresponding":false},{"id":1557218,"name":"David A Brow","orcid":null,"position":6,"is_corresponding":false},{"id":1557213,"name":"Eric J Montemayor","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution","abstract":"The spliceosome is a dynamic assembly of five small nuclear ribonucleoproteins (snRNPs) that removes introns from eukaryotic pre-mRNA. U6, the most conserved of the spliceosomal small nuclear RNAs (snRNAs), participates directly in catalysis. Here, we report the crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein. It reveals a unique interlocked RNP architecture that sequesters the 5' splice site-binding bases of U6 snRNA. RRMs 1, 2 and 4 of Prp24 form an electropositive groove that binds double-stranded RNA and may nucleate annealing of U4 and U6 snRNAs. Substitutions in Prp24 that suppress a mutation in U6 localize to direct RNA-protein contacts. Our results provide the most comprehensive view to date of a multi-RRM protein bound to RNA and reveal striking coevolution of protein and RNA structure.","is_dataset_classified":null,"base_score":4.356708826689592,"endowment":4.356708826689592,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24837192","pmcid":"PMC4141773","openalex_id":"https://openalex.org/W2029751805","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM065166","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065166","title":null},{"funder_name":"National Institutes of Health","grant_id":"4R01GM065166-13","title":"Structure and Function of U6 Spliceosomal RNA"}],"total_grants":3,"fwci":4.4186,"citation_percentile":0.95539187,"influential_citations":0,"citation_trend":[{"year":2014,"count":3},{"year":2015,"count":14},{"year":2016,"count":9},{"year":2017,"count":8},{"year":2018,"count":9},{"year":2019,"count":7},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":8},{"year":2024,"count":8},{"year":2025,"count":3},{"year":2026,"count":2}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4141773","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4141773","host_type":"repository"},{"url":"http://www.nature.com/articles/nsmb.2832.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nsmb.2832","host_type":"publisher"},{"url":"https://doi.org/10.1038/nsmb.2832","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24837192","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4141773","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4141773?pdf=render","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/pmc4141773?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1038/nsmb.2832","host_type":""}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","RNA modifications and cancer","0301 basic medicine","0303 health sciences","03 medical and health sciences","Binding Sites","Crystallography, X-Ray","Evolution, Molecular","Models, Molecular","Protein Structure, Tertiary","RNA, Small Nuclear","Ribonucleoprotein, U4-U6 Small Nuclear","Ribonucleoproteins, Small Nuclear","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins"],"mesh_terms":["Binding Sites","Models, Molecular","RNA, Small Nuclear","Saccharomyces cerevisiae","Ribonucleoproteins, Small Nuclear","Ribonucleoprotein, U4-U6 Small Nuclear","Protein Structure, Tertiary","Crystallography, X-Ray","Evolution, Molecular","Saccharomyces cerevisiae Proteins"],"keywords":["snRNP","Small nuclear RNA","Spliceosome","Prp24","Small nuclear ribonucleoprotein","RNA splicing","RNA","Ribonucleoprotein","Intron","Ribozyme","Biology","RNA-binding protein","Ribonucleoprotein particle","Minor spliceosome","Cell biology","Genetics","Biophysics","Non-coding RNA","Gene","Models, Molecular","Binding Sites","Saccharomyces cerevisiae Proteins","Ribonucleoprotein, U4-U6 Small Nuclear","Saccharomyces cerevisiae","Crystallography, X-Ray","Ribonucleoproteins, Small Nuclear","Protein Structure, Tertiary","Evolution, Molecular","RNA, Small Nuclear"],"sdg_mappings":[{"sdg_number":2,"sdg_label":"2. Zero hunger"},{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:47:59.845896Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}