{"doi":"10.1038/nsmb.1577","title":"Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome","abstract":null,"journal":"Nature Structural & Molecular Biology","year":2009,"id":663715,"datarank":0.8882367629465725,"base_score":5.921578419643816,"endowment":5.921578419643816,"self_citation_contribution":0.8882367629465725,"citation_network_contribution":0.0,"self_endowment_contribution":0.8882367629465725,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":372,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1332623,"name":"Albert Weixlbaumer","orcid":"0000-0001-9635-3844","position":1,"is_corresponding":false},{"id":1732930,"name":"David Loakes","orcid":null,"position":2,"is_corresponding":false},{"id":1732931,"name":"Ann C Kelley","orcid":null,"position":3,"is_corresponding":false},{"id":1732932,"name":"V Ramakrishnan","orcid":null,"position":4,"is_corresponding":false},{"id":1732929,"name":"Rebecca M Voorhees","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome","abstract":"Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.","is_dataset_classified":null,"base_score":5.921578419643816,"endowment":5.921578419643816,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19363482","pmcid":"PMC2679717","openalex_id":"https://openalex.org/W2152425069","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"MC_U105184332","title":null},{"funder_name":"Wellcome Trust","grant_id":"082086","title":"Structural studies on ribosomes."},{"funder_name":"Medical Research Council","grant_id":"U.1051.04.018(78935)","title":null},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"}],"total_grants":4,"fwci":15.1209,"citation_percentile":0.99470967,"influential_citations":0,"citation_trend":[{"year":2012,"count":32},{"year":2013,"count":31},{"year":2014,"count":31},{"year":2015,"count":35},{"year":2016,"count":21},{"year":2017,"count":19},{"year":2018,"count":20},{"year":2019,"count":11},{"year":2020,"count":22},{"year":2021,"count":14},{"year":2022,"count":10},{"year":2023,"count":13},{"year":2024,"count":9},{"year":2025,"count":10},{"year":2026,"count":5}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2679717","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2679717","host_type":"repository"},{"url":"http://www.nature.com/articles/nsmb.1577.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nsmb.1577","host_type":"publisher"},{"url":"https://doi.org/10.1038/nsmb.1577","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19363482","host_type":"repository"},{"url":"https://resolver.caltech.edu/CaltechAUTHORS:20180116-155008633","host_type":"repository"},{"url":"https://www.dora.lib4ri.ch/psi/islandora/object/psi%3A17054","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2679717","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2679717?pdf=render","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/pmc2679717?pdf=render","host_type":""},{"url":"http://dx.doi.org/10.1038/nsmb.1577","host_type":""},{"url":"https://dx.doi.org/10.1038/nsmb.1577","host_type":""},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2679717/","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/nsmb.1577","host_type":""}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","Peptidase Inhibition and Analysis","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Escherichia coli","Peptidyl Transferases","Protein Binding","Ribosomal Proteins","Ribosomes","RNA, Ribosomal, 23S","RNA, Transfer","Substrate Specificity","Thermus thermophilus","Protein Structure, Secondary","Crystallography, X-Ray","Static Electricity"],"keywords":["Peptidyl transferase","50S","Ribosome","Transfer RNA","P-site","A-site","Protein subunit","Translation (biology)","Chemistry","Eukaryotic Ribosome","Ribosomal RNA","Ribosomal protein","Internal ribosome entry site","Biochemistry","Biophysics","Biology","Binding site","RNA","Messenger RNA","Ribosomal Proteins","570","Thermus thermophilus","Static Electricity","Crystallography, X-Ray","Article","Protein Structure, Secondary","Substrate Specificity","RNA, Ribosomal, 23S","RNA, Transfer","Peptidyl Transferases","Escherichia coli","Ribosomes","Protein Binding"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T23:14:11.942330Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}