{"doi":"10.1038/nprot.2008.73","title":"Analyzing real-time PCR data by the comparative CT method","abstract":"Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.","journal":"Nature Protocols","year":2008,"id":1608,"datarank":21.10802124060917,"base_score":10.1810435286889,"endowment":10.1810435286889,"self_citation_contribution":1.5271565293033351,"citation_network_contribution":19.580864711305832,"self_endowment_contribution":1.5271565293033351,"citer_contribution":19.580864711305832,"corpus_percentile":98.8,"corpus_rank":195,"citation_count":26397,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2008-06-01","authors":[{"id":12216,"name":"Kenneth J. Livak","orcid":"0000-0001-9105-5856","position":1,"is_corresponding":false},{"id":18583,"name":"Thomas D. Schmittgen","orcid":"0000-0002-2812-8546","position":0,"is_corresponding":true}],"reference_count":23,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}