{"doi":"10.1038/npre.2007.819.1","title":"CARE1, a TY3-gypsy long terminal repeat retrotransposon in the food legume chickpea (Cicer arietinum L)","abstract":"<jats:title>Abstract</jats:title><jats:p>We report a novel Ty3-gypsy long terminal repeat retrotransposon CARE1 (<jats:italic>Cicer arietinum</jats:italic> retro-element 1) in chickpea. This 5920-bp AT-rich (63%) element carries 723-bp 5' and 897-bp 3' LTRs respectively flanking an internal region of 4300-bp. The LTRs of CARE1 show 93.9% nucleotide identity to each other and have 4-bp (ACTA) terminal inverted repeats. A 17-bp potential tRNAmet primer binding site downstream to 5' LTR and a 13-bp polypurine tract upstream to 3' LTR have been identified. The order of domains (Gag-proteinase-reverse transcriptase-RNaseH-integrase) in the deduced amino acid sequence and phylogenetic tree constructed using reverse transcriptase sequences places CARE1 in the gypsy group of retrotransposons. Homologues of a number of <jats:italic>cis</jats:italic>-elements including CCAAT, TATA and GT-1 have been detected in the regulatory region or the 5' LTR of CARE1. Transgenic tobacco plants containing 5' LTR:GUS construct show that its 5'-LTR is inactive in a heterologous system under normal as well as tissue culture conditions. Genomic Southern blot experiments using 5’LTR of the element as a probe show that CARE1 or its related elements are present in the genomes of various chickpea accessions from various geographic regions.</jats:p>","journal":"Nature Precedings","year":2007,"id":15790,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":119083,"name":"Kailash Upadhyaya","orcid":null,"position":1,"is_corresponding":false},{"id":119082,"name":"Manoj Rajput","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W113856995","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.01317741,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1038/npre.2007.819.1","host_type":"journal"},{"url":"https://doi.org/10.1038/npre.2007.819.1","host_type":"GOLD"},{"url":"https://doi.org/10.1038/npre.2007.819.1","host_type":"publisher"},{"url":"https://www.nature.com/articles/npre.2007.819.1.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/npre.2007.819.1","host_type":"publisher"},{"url":"http://precedings.nature.com/documents/819/version/1","host_type":"journal"}],"fields_of_study":["Legume Nitrogen Fixing Symbiosis","Chromosomal and Genetic Variations","Plant Virus Research Studies","Biology","Agricultural and Food Sciences","Environmental Science"],"mesh_terms":[],"keywords":["Retrotransposon","Long terminal repeat","Primer binding site","Reverse transcriptase","Biology","Genetics","Primer (cosmetics)","Phylogenetic tree","Genome","Gene","Transposable element","RNA","Chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T18:58:10.610385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}