{"doi":"10.1038/npg.els.0000997","title":"Northern Blotting and RNA Detection","abstract":"<jats:title>Abstract</jats:title>\n          <jats:p>Northern blotting is a means of identifying a certain RNA species in a complex mixture of RNA. Commonly used to evaluate qualitatively and quantitatively the expression of a gene, Northern blotting involves isolation of RNA, size fractionation of the denatured RNA through gel electrophoresis, transfer of the separated RNA to a membrane, hybridization with a specific probe, and detection.</jats:p>","journal":"Encyclopedia of Life Sciences","year":2001,"id":34502,"datarank":0.10640668494959492,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0024346078656031136,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0024346078656031136,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":178623,"name":"Teresa Zimmers‐Koniaris","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24523987","pmcid":null,"openalex_id":"https://openalex.org/W1937235861","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2020,"count":1}],"oa_status":"closed","license":"http://doi.wiley.com/10.1002/tdm_license_1.1","oa_locations":[{"url":"https://pericles-gcp.literatumonline.com/doi/pdf/10.1038/npg.els.0000997","host_type":"publisher"},{"url":"https://pericles-gcp.literatumonline.com/doi/full-xml/10.1038/npg.els.0000997","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1038/npg.els.0000997","host_type":"publisher"},{"url":"http://doi.org/10.1038/npg.els.0000997","host_type":"journal"}],"fields_of_study":["Molecular Biology Techniques and Applications","RNA and protein synthesis mechanisms","RNA modifications and cancer","Chemistry","Biology"],"mesh_terms":[],"keywords":["Blot","RNA","Northern blot","Molecular biology","Messenger RNA","Southern blot","Gel electrophoresis","RNA extraction","Gene expression","Biology","Gene","Chemistry","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T18:52:56.049059Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}