{"doi":"10.1038/ncomms6164","title":"STIM1 triggers a gating rearrangement at the extracellular mouth of the ORAI1 channel","abstract":null,"journal":"Nature Communications","year":2014,"id":673391,"datarank":0.6646225198264971,"base_score":4.430816798843313,"endowment":4.430816798843313,"self_citation_contribution":0.6646225198264971,"citation_network_contribution":0.0,"self_endowment_contribution":0.6646225198264971,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":83,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":763092,"name":"Ariel Quintana","orcid":"0009-0009-7032-6042","position":1,"is_corresponding":false},{"id":139030,"name":"Yubin Zhou","orcid":"0000-0001-7962-0517","position":2,"is_corresponding":false},{"id":1759388,"name":"Nupura Hirve","orcid":null,"position":3,"is_corresponding":false},{"id":1759390,"name":"Sahasransu Mahapatra","orcid":null,"position":4,"is_corresponding":false},{"id":486329,"name":"Patrick G. Hogan","orcid":"0000-0003-3196-9618","position":5,"is_corresponding":false},{"id":1759385,"name":"Aparna Gudlur","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"STIM1 triggers a gating rearrangement at the extracellular mouth of the ORAI1 channel","abstract":"The ER-resident regulatory protein STIM1 triggers store-operated Ca(2+) entry by direct interaction with the plasma membrane Ca(2+) channel ORAI1. The mechanism of channel gating remains undefined. Here we establish that STIM1 gates the purified recombinant ORAI1 channel in vitro, and use Tb(3+) luminescence and, separately, disulfide crosslinking to probe movements of the pore-lining helices. We show that interaction of STIM1 with the cytoplasmic face of the human ORAI1 channel elicits a conformational change near the external entrance to the pore, detectable at the pore Ca(2+)-binding residue E106 and the adjacent pore-lining residue V102. We demonstrate that a short nonpolar segment of the pore including V102 forms a barrier to ion flux in the closed channel, implicating the STIM1-dependent movement in channel gating. Our data explain the close coupling between ORAI1 channel gating and ion selectivity, and open a new avenue to dissect the gating, modulation and inactivation of ORAI-family channels.","is_dataset_classified":null,"base_score":4.430816798843313,"endowment":4.430816798843313,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25296861","pmcid":null,"openalex_id":"https://openalex.org/W2070453934","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"5R01AI084167-07","title":"Regulation of store-operated Ca2+ entry: STIM and ORAI"}],"total_grants":1,"fwci":5.1351,"citation_percentile":0.97063052,"influential_citations":0,"citation_trend":[{"year":2015,"count":10},{"year":2016,"count":12},{"year":2017,"count":12},{"year":2018,"count":7},{"year":2019,"count":11},{"year":2020,"count":13},{"year":2021,"count":8},{"year":2022,"count":2},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":3}],"oa_status":"bronze","license":"Springer Nature TDM","oa_locations":[{"url":"https://www.nature.com/articles/ncomms6164.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/ncomms6164.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/ncomms6164","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncomms6164","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25296861","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4376667","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.797.9166","host_type":""},{"url":"http://dx.doi.org/10.1038/ncomms6164","host_type":""},{"url":"https://dx.doi.org/10.1038/ncomms6164","host_type":""}],"fields_of_study":["Ion Channels and Receptors","Ion channel regulation and function","Neurobiology and Insect Physiology Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Stromal Interaction Molecule 1","ORAI1 Protein","Calcium","Cell Membrane","Humans","Membrane Proteins","Neoplasm Proteins","Calcium Channels","Ion Channel Gating","Protein Structure, Secondary"],"keywords":["Gating","ORAI1","Biophysics","Ion channel","Chemistry","Patch clamp","TRPC1","Coupling (piping)","Extracellular","STIM1","Cell biology","Membrane","Biochemistry","Materials science","Biology","Receptor","ORAI1 Protein","Cell Membrane","Membrane Proteins","Article","Protein Structure, Secondary","Neoplasm Proteins","Humans","Calcium","Calcium Channels","Stromal Interaction Molecule 1","Ion Channel Gating"],"sdg_mappings":[{"sdg_number":2,"sdg_label":"2. 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