{"doi":"10.1038/ncomms12622","title":"Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Eukaryotic mRNAs often contain a Kozak sequence that helps tether the ribosome to the AUG start codon. The mRNA of histone H4 (\n                    <jats:italic>h4</jats:italic>\n                    ) does not undergo classical ribosome scanning but has evolved a specific tethering mechanism. The cryo-EM structure of the rabbit ribosome complex with mouse\n                    <jats:italic>h4</jats:italic>\n                    shows that the mRNA forms a folded, repressive structure at the mRNA entry site on the 40S subunit next to the tip of helix 16 of 18S ribosomal RNA (rRNA). Toe-printing and mutational assays reveal that an interaction exists between a purine-rich sequence in\n                    <jats:italic>h4</jats:italic>\n                    mRNA and a complementary UUUC sequence of helix h16. Together the present data establish that the\n                    <jats:italic>h4</jats:italic>\n                    mRNA harbours a sequence complementary to an 18S rRNA sequence which tethers the mRNA to the ribosome to promote proper start codon positioning, complementing the interactions of the 40S subunit with the Kozak sequence that flanks the AUG start codon.\n                  </jats:p>","journal":"Nature Communications","year":2016,"id":45368,"datarank":1.3692857463985408,"base_score":4.0943445622221,"endowment":4.0943445622221,"self_citation_contribution":0.6141516843333151,"citation_network_contribution":0.7551340620652257,"self_endowment_contribution":0.6141516843333151,"citer_contribution":0.7551340620652257,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":39,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":212019,"name":"Jean-François Ménétret","orcid":null,"position":1,"is_corresponding":false},{"id":212020,"name":"Angelita Simonetti","orcid":null,"position":2,"is_corresponding":false},{"id":138179,"name":"Alexander G. Myasnikov","orcid":null,"position":3,"is_corresponding":false},{"id":60582,"name":"Quentin Vicens","orcid":"0000-0003-3751-530X","position":4,"is_corresponding":false},{"id":138904,"name":"Lydia Prongidi-Fix","orcid":null,"position":5,"is_corresponding":false},{"id":212021,"name":"S. Kundhavai Natchiar","orcid":null,"position":6,"is_corresponding":false},{"id":138186,"name":"Bruno P. Klaholz","orcid":null,"position":7,"is_corresponding":false},{"id":138905,"name":"Gilbert Eriani","orcid":null,"position":8,"is_corresponding":false},{"id":60324,"name":"Franck Martin","orcid":"0009-0002-8598-8443","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.0943445622221,"endowment":4.0943445622221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27554013","pmcid":"PMC4999511","openalex_id":"https://openalex.org/W2507683869","authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"243296","title":"Integrative structure and function study of the bacterial and human protein synthesis machinery."}],"total_grants":1,"fwci":4.1004,"citation_percentile":0.94290697,"influential_citations":1,"citation_trend":[{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":10},{"year":2019,"count":7},{"year":2020,"count":7},{"year":2021,"count":5},{"year":2022,"count":6},{"year":2023,"count":3},{"year":2024,"count":7},{"year":2025,"count":6},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/ncomms12622.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/ncomms12622.pdf","host_type":"GOLD"},{"url":"https://www.nature.com/articles/ncomms12622.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/ncomms12622","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncomms12622","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27554013","host_type":"repository"},{"url":"https://hal.science/hal-02294260","host_type":"repository"},{"url":"https://doaj.org/article/13bf703b19f34a32b972880d5c0ed24e","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4999511","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4999511","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4999511?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1038/ncomms12622","host_type":""},{"url":"https://dx.doi.org/10.1038/ncomms12622","host_type":""},{"url":"https://hal.science/hal-02294260v1","host_type":""},{"url":"https://hal.science/hal-02294260v1/document","host_type":""}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","RNA Research and Splicing","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Base Pairing","Base Sequence","Codon, Initiator","Histones","Mice","Models, Molecular","Mutation","Nucleic Acid Conformation","Peptide Chain Initiation, Translational","RNA, Messenger","RNA, Ribosomal, 18S","Rabbits","Ribosomes"],"mesh_terms":["Animals","Base Sequence","Histones","Models, Molecular","Mutation","Nucleic Acid Conformation","Peptide Chain Initiation, Translational","Rabbits","Ribosomes","RNA, Messenger","RNA, Ribosomal, 18S","Codon, Initiator","Base Pairing","Mice"],"keywords":["Shine-Dalgarno sequence","Ribosomal binding site","Eukaryotic Ribosome","Start codon","Ribosomal RNA","Ribosome","Messenger RNA","Eukaryotic Small Ribosomal Subunit","Biology","Translation (biology)","Translational frameshift","30S","Eukaryotic translation","Internal ribosome entry site","Genetics","Five prime untranslated region","RNA","Cell biology","Gene","Models, Molecular","Base Sequence","Science","Q","Codon, Initiator","[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology","Article","[SDV] Life Sciences [q-bio]","Histones","Mice","Mutation","[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology","RNA, Ribosomal, 18S","Animals","Nucleic Acid Conformation","RNA, Messenger","Rabbits","Peptide Chain Initiation, Translational","Base Pairing","Ribosomes"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"emdb"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-03T01:04:46.165913Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}